5. Analysis of Cell Components

Question 1

what allows you to see organelles up close?

Answer 1

Microscopes

Question 2

define magnification

Answer 2

how much bigger the image is then the specimen

Question 3

what is the magnification equation

Answer 3

Magnification= Size of image / size of real object

Question 4

define resolution

Answer 4

how well a microscope distinguishes between two points that are close together

Question 5

name the two types of microscopes

Answer 5

• optical
• electron

Question 6

what are the features of an optical microscope

Answer 6

• use light to form an image
• 0.2 um max resolution
• x1500 max magnification

Question 7

what are the features of an electron microscope

Answer 7

• use electrons to form an image
• have a higher resolution than optical microscopes
• 0.0002 max resolution
• x 1 500 000 max magnification

Question 8

what are the two types of electron microscopes

Answer 8

• transmission electron microscope (TEM)
• scanning electron microscope (SEM)

Question 9

TEMs use ——— to focus a beam of electrons, which is then transmitted through the specimen

Answer 9

electromagnets

Question 10

TEMs can only be used on —- specimens

Answer 10

thin

Question 11

list the features of the TEM

Answer 11

• high resolution images
• darker image, as the specimen absorbs more electrons
• you can see internal structures such as chloroplasts

Question 12

SEMS can be used on —– specimens

Answer 12

thick

Question 13

SEMs scan a beam of electrons across the specimen, knocking off electrons, which gather in the ——- —- ——, which then forms an ——.

Answer 13

cathode ray tube
image

Question 14

list the features of an SEM

Answer 14

• give a lower resolution than TEMs
• can produce 3D images