4.Pred Flashcards

1
Q

Faze uzgoja?

A

Lag,log,stacionarna, odumiranje

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2
Q

Stres stanica?

A

Oksidansi, izgladnjivanje, osmotski sok,pH vrijednosti temp,

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3
Q

Stanicni odgovor na stres

A

Fenotip: kapsula, popravak oštećenja, propusnost
Genotip: mutacije

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4
Q

Vrste odgovara na stres:

A
  1. Chaperone
    2.transkripcijska regulacija
    3.quorum sensing
  2. Latentni stanje
  3. Heat shock
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5
Q

Chaperone?

A
  • proteini koji osiguravaju pravilnuu konformaciju drugih proteina
  • sprječavaju dentaruaciju protein
  • produkti denaturacije aktiviraju chaperone
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6
Q

Transkripcijska regulacija

A

Regulacija sinteze mRNA

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7
Q

Quorom sensing

A
  1. Formiranje odgovora s obzirom na gustoću populacije
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8
Q

Latentni stanje

A
  1. Usporava se metabolizam
  2. Smanjuje se transport tvari
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9
Q

Heat shock

A

Kontroliran sigma 32 faktorom
Smanjivanje oštećenja proteinA uslijed povišene temperature
Aktivacija chaperona jer smanjuju denaturacije

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10
Q

Protein RpoS

A

Regulacija 500 gena
- odgovor na stres
-promjene u stanici

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11
Q

Promjene u stacionarnoj fazi

A
  1. Manje stanice
  2. Otpornija stijenka
  3. Kondenzacija nukleoida
  4. Smanjenje sinteze proteina
  5. Promjene globalnih regulatora
  6. Povećana Sinteza proteza
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12
Q

Gasp

A

Jaki
Slabi
Abortivni
Umjereni

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