4E- Generation of ATP from Glucose Flashcards
What are the general steps of glycolysis?
Where does glycolysis take place?
cytosol
Which steps use ATP?
steps 1 and 3
Which steps generate ATP?
7 and 10
remember that these gain 2 ATP at these steps cuz they are using 2 molceules rather than 1 for the ATP-using steps
What step generates NADH?
Step 6
What is the fate of pyruvate and NADH during aerobic conditions?
the 2 pyruvatees produced get converted into acetyl-CoA and enter the TCA cycle
the NADH gets used in the electron transport chain
What is the fate of pyruvate and NADH during anaerobic conditions?
the pyruvates and the NADH get both used by lactate dehydrogensase and get converted into lactate
How many ATPs are produced in the aerobic vs anaerobic conditions?
aerobic:
- CAC Summary:
2 x 3 NADH
2 x 1 FADH2
2 x 1 GTP
Pyruvate Dehydrogenase Summary:
2 x 1 NADH
Glycolysis Summary:
2 ATP
2 NADH
* Total of 4 ATP, 10NADH2 (2.5 ATP each), 2 FADH2 (1.5 each) = 32 ATP
anaerobic
only glycolysis works –> 2 ATP
What are the types of tissues that are dependent on anaerobic glycolysis?
tissues that have a low ATP demand, high levels of glycolytic enzymes and few capillaries.
Why is the eye dependent on anaerobic glycolysis?
cuz it contains cells that transmit light, so it cant be filled with mitochondria as it would block the light
this is especially present in the lens
What is the cori cycle?
lactate is removed from the tissues and converted back to pyruvate
What is the rate-limitind step of glycolysis?
PFK-1
it is regulated by many factors
Describe the regulation of the glycolytic pathway is regulated, with particular reference to the roles of phosphfructokinase-1 (PFK-1), phosphfructokinase-2 (PFK-2), and fructose 2,6 – bisphosphate, and ATP, ADP, and AMP
What is a major cause of lactic acidemia?
from an increased NADH concentration in the blood, as it prvents pyruvate oxidation in the TCA and it directs pyruvate –> lactate
