4A: DNA, RNA and Protein Synthesis Flashcards

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1
Q

How is DNA store in Eukaryotic Cells?

A

Wound around histones into chromosomes

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2
Q

How is DNA stored in Prokaryotic Cells?

A

a circular chromosome that is supercoiled to fit in the cell

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3
Q

What is a Gene?

A

a coding section of DNA that codes for a specific amino acid sequence forming a polypeptide chain

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4
Q

What is a Locus?

A

the fixed position where each gene can be found on a chromosome

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5
Q

What is non-coding DNA?

A

DNA that does not code for polypeptides called introns

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6
Q

Where are introns found?

A

Between exons in Eukaryotic DNA

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7
Q

What are Alleles?

A

different forms of the same gene

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8
Q

What are Homologous Chromosomes?

A

a pair of chromosomes that are the same size and have the same genes in the same locus

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9
Q

What is protein Synthesis?

A

the production of proteins from the information contained within a cell’s DNA

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10
Q

What is Transcription?

A

where the DNA code is copied into a molecule called mRNA

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11
Q

What is Translation?

A

where the mRNA joins with an organelle called a Ribosome and the code it carried is used to synthesise a protein

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12
Q

What is RNA?

A

a single polynucleotide strand used in Protein Synthesis

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13
Q

What is mRNA?

A

made during transcription, carries the genetic code from DNA to Ribosomes

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14
Q

What is tRNA?

A

carries Amino Acids that are used to make proteins to the Ribosomes

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15
Q

Describe Transciption

A
  1. mRNA associates with a ribosome / ribosome attaches to mRNA;
    Idea of association is required
  2. Ribosome moves to / finds the start codon / AUG;
  3. tRNA brings / carries (appropriate / specific) amino acid;
    Must be explicitly stated and not inferred.
  4. Anticodon (on tRNA complementary) to codon (on mRNA);
  5. Ribosome moves along to next codon;
    OR
    Ribosome ‘fits’ around two codons / can fit two tRNAs;
    Must be explicitly stated and not inferred.
  6. (Process repeated and) amino acids join by peptide bonds /
    condensation reaction (to form polypeptide);
    OR
    (Process repeated and) amino acids joined using (energy from) ATP
    (to form polypeptide);
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16
Q

Describe Translation

A
  • mRNA attaches to ribosome
  • codon on mRNA
  • binds to an anti-codon on tRNA
  • each tRNA brings a specific amino acid
  • sequence of codons/bases on mRNA determines the order of amino acids
  • formation of peptide bonds OR amino acids joined by condensation reactions
17
Q

Describe the function of Ribosomes in Protein Synthesis

A
  • Translation/construction of polypeptide(s)
  • Provide a binding site for mRNA OR mRNA attaches to ribosome
  • Provides binding sites for (two) tRNA molecules
  • Two amino acids are held close together
  • Formation of peptide bond(s)
  • (allows) assembly of amino acids into the sequence/primary structure
18
Q

Describe the role of tRNA in Translation

A
  • tRNA attaches to (specific) amino acids
  • tRNA (with amino acid) moves to the ribosome
  • The anticodon of tRNA binds to/forms hydrogen bonds with (a complementary) codon of mRNA
19
Q

Describe the role of ATP in Translation

A
  • It activates the tRNA with an amino acid OR binds a specific amino acid with tRNA
  • The phosphorylation of tRNA OR creates a high energy bond which is transferred to the tRNA
20
Q

How is pre-mRNA turned into mRNA?

A

Introns are removed and Exons are spliced together before it exits nuclear envelope

21
Q

What are three characteristics of the genetic code?

A

Degenerate
Non-overlapping
Universal

22
Q

What does degenerate mean?

A

there are more possible triplet combinations that amino acids to be coded for

23
Q

What does non-overlapping mean?

A

triplets don’t share bases

24
Q

What does universal mean?

A

same specific base triplets code for the same amino acids in all living things