4.6 LEC

Question 1

Method where neither the probe nor the target is immobilized?

Answer 1

Solution hybridization

Question 2

Type of analysis often measured using solution hybridization?

Answer 2

mRNA expression

Question 3

Variation of solution hybridization involving a probe attached to beads?

Answer 3

Bead hybridization

Question 4

Type of probe used to detect RNA hybrids in solution hybridization?

Answer 4

Detection probe.

Question 5

Assay indicating binding by a change in mobility, typically slower migration?

Answer 5

Gel mobility shift assay

Question 6

Factors that bind to cis-acting elements in gene regulation identified by solution hybridization?

Answer 6

Trans factors

Question 7

Advantage of hybridization methods in clinical applications?

Answer 7

Direct discovery of molecular genetic information.

Question 8

Technique used to determine the start or termination point of transcripts?

Answer 8

S1 mapping

Question 9

Method that is more sensitive than northern blotting due to reduced target loss?

Answer 9

S1 mapping

Question 10

Assay capturing DNA probe target hybrids on a solid support?

Answer 10

Sandwich assay.

Question 11

Biotinylated probe that binds to streptavidin in sandwich assays?

Answer 11

Capture probe