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RESET - MOLBIO PRELIMS > 2. LAB > Flashcards

2. LAB Flashcards

1
Q

What are tools used to measure extremely small volumes of liquid?

A

Micropipettes

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2
Q

What defines the accuracy of a micropipette?

A

Defined range of volume

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3
Q

What is the principle of operation for micropipettes?

A

Air displacement

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4
Q

What is the smallest volume a P10 micropipette can aspirate?

A

0.2 µL

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5
Q

What is the volume range for a P10 micropipette?

A

0.5-10 µL

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6
Q

What is the volume range for a P20 micropipette?

A

2-20 µL

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7
Q

What is the volume range for a P200 micropipette?

A

20-200 µL

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8
Q

What is the volume range for a P1000 micropipette?

A

200-1000 µL

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9
Q

Parts of Micropipettes

A

PUSH BUTTON
TIP EJECTOR BUTTON
TIP EJECTOR COLLAR
VOLUMETER

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10
Q

What part is used for aspiration or dispensing the fluid?

A

Push button

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11
Q

What part removes the micropipette tip after use?

A

Tip ejector button

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12
Q

What part pushes down the micropipette tip?

A

Tip ejector collar

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13
Q

Where are the dial windows or numbers located?

A

Volumeter

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14
Q

What instrument is used to measure the pH of a solution?

A

pH meter

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15
Q

What are the two types of measuring probes in a pH meter?

A

Glass electrode and reference electrode

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16
Q

What is measured by a pH meter?

A

Concentration of hydrogen ions

17
Q

What is the standard buffer used in molecular biology for pH 7.0 (±0.02)?

A

Buffer solution pH 7.0

18
Q

What is the primary use of a buffer solution in pH meters?

A

Calibration buffer

19
Q

What types of buffer solutions are used for pH calibration?

A

Neutral pH 7, Acidic pH 4, Alkaline pH 10

20
Q

How does a pH meter function?

A

Measures voltage between two electrodes

RESET - MOLBIO PRELIMS (16 decks)

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