4.3 Transcription

Question 1

Which strand is the Template and Non-Template strand

Answer 1

strand that mRNA is added is template the other strand is non-template

Question 2

What are some differences between DNA and RNA

Answer 2

DNA- had T enzyme and goes from 5 prime to 3 prime
RNA- has U enzyme and goes from 3 prime to 5 prime

Question 3

Explain Alternative Splicing

Answer 3

it is where multiple mRNA’s is made out of the same gene.

Question 4

List all of the enzymes in an eukaryotic gene

Answer 4

Promoter, enhancer, silencer, exons, introns

Question 5

What are the stages of transcription in order.

Answer 5

Initiation-elongation-termination

Question 6

Where does transcription start.

Answer 6

TATA box

Question 7

Explain stage 2

Answer 7

mRNA break free and a 5 prime cap is attached. RNA polymerase copies and splicing occur.

Question 8

Explain stage 1

Answer 8

TF’s bind to promoter to prepare the RNA polymerase to start copying the mRNA

Question 9

Explain stage 3

Answer 9

PA site it cut out and a polyA tail is added to make new RNA molecule