4.1 enzymes Flashcards
enzymes
catalysts, reduce activation energy required for chemical reactions
substrate
reactant which binds to enzyme
active site
where substrate fits into enzyme
Reaction specific
each enzyme has a specific substrate
Not consumed in reaction
single enzyme can do lots of reactions
Enzymes are made of…
protein
Affected by cellular conditions
can be denatured
How do you name enzymes?
Reactions they catalyze, most end in -ase
Induced Fit Model
Enzyme fits around substrate, less activation energy needed
Enzyme Concentration
as ^ enzyme ^ reaction rate, more collisions, reaction rate levels off, substrate is a limiting factor
Substrate Concentration
As ^ substrate ^ reaction rate, enzyme is limiting factor
Temperature
Optimum Temp is 37 degrees Celsius, greatest # of successful collisions, human enzymes 35-40
pH
pepsin (stomach) 2.3, trypsin (small intestine) 8
ON
maintain/change active site shape, activator/cofactors are inorganic ions, coenzymes are vitamins
OFF
inhibitors reduce enzyme activity, competitive compete with substrate, noncompetitive change shape to prevent binding