4 Kinetics of Substrate Utilization, Product Formation, & Biomass Production In Cell Cultures

Question 1

Cellular representations are multicomponent

Answer 1

Structured

Question 2

Single component representations

Answer 2

Unstructured

Question 3

Considers discrete, heterogeneous individual cells

Answer 3

Segregated

Question 4

Considers average cellular properties

Answer 4

Unsegregated

Question 5

All cellular components must be produced in production to their abundances

Answer 5

Balanced growth

Question 6

Generally, ________, ________, and empirical modesls are used to describe cell growth rate.

Answer 6

Unsegregated & unstructured

Question 7

It is difficult to obtain useful kinetic information on microbial cell growth from non-uniform condition reactors. Hence it is desirable to study kinetics in reactors that are ideal.

Answer 7

True

Question 8

[Ideal Batch Reactor]
To get the “growth” or accumulation of any component i (product biomass) in the reactor:

Answer 8

d/dt (culture volume) (mole conc.of component i)

d/dt (V) (Ci) = V (ri)

Question 9

If no liquid is added to or removed from the reactor and if gas stripping of
culture liquid is negligible

Answer 9

d (ci)/ dt = ri

Direct measurements of the change in conc. of the component i gives us the rate of its formation

Question 10

[Ideal CSTR]

Steady state conditions:

Answer 10

Rate of addition to reactor - Rate of removal from reactor + Rate of formation within the reactor = 0

Question 11

Reciprocal of mean holding/residence time

Answer 11

Dilution rate, D

Question 12

Property of CSTR

Answer 12

• Kinetics is more straightforward
• Can adjust to steady environment &achieve or closely approx a state of balanced growth

Question 13

u (myu)

Answer 13

specific cell growth rate of the cells (1/time)

Question 14

If xi=0, the liquid stream feed consist of sterile nutrients.

Answer 14

x cannot be determined

Dxi = (D-u) x

Question 15

Nonzero cell population can be maintained when D=u

Answer 15

True

Question 16

Kinetic models for growth cells can be done knowing substrates can be….

Answer 16

growth-limiting

Question 17

Functional relationship between. The specific growth rate, u, & essential compound’s conc..

Answer 17

Monod Growth Kinetics

Question 18

Who introduced classification of product formation kinetics for Batch Reactor Fermentation?

Answer 18

F.H. Deindoerfer

Question 19

[Classification of Fermentation Patterns]
Nutrients converted to products in a fixed stoichiometry without accumulation of intermediates

Answer 19

Simple

Question 20

[Classification of Fermentation Patterns for Batch Reactor]

Nutrients converted to products in variable stoichiometry proportion without accumulation of intermediates

Answer 20

Simultaneous

Question 21

[Classification of Fermentation Patterns for Batch Reactor]

Nutrients converted to product with accumulation of an intermediate

Answer 21

Consecutive

Question 22

[Classification of Fermentation Patterns for Batch Reactor]

Nutrients completely converted to intermediate before conversion to product, or selectively converted to product in preferential order.

Answer 22

Stepwise