4 Enzymes Flashcards

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1
Q

what is an enzyme?

A
  1. BIOLOGICAL CATALYST - speeds up the rate of a particular reaction, remain unchanged and repeatedly catalyse a reaction
  2. GLOBULAR PROTEIN - a specific sequence of amino acids fold up to form a three-dimensional shape
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2
Q

what is a substrate?

A

REACTANTS IN AN ECR, interact with enzyme and undergo a reaction by forming a SUBSTRATE-ENZYME COMPLEX

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3
Q

what is a product?

A

MOLECULE(S) PRODUCED FROM THE SUBSTRATE AT THE END OF AN ECR

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4
Q

why are enzymes necessary to life?

A

CHEMICAL REACTIONS DO NOT TAKE PLACE AT A RATE SUITABLE FOR LIFE UNDER THE TEMP. RANGE IT EXISTS AT,
Es SPEED UP RATE OF REACTION AND ALLOW FINE CONTROL OVER ALL REACTIONS AND RATES OF REACTION

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5
Q

what is metabolism?

A

SUM OF ALL DIFFERENT REACTIONS AND REACTION PATHWAYS IN A CELL OR AN ORGANISM

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6
Q

what are anabolic reactions?

A

REACTIONS THAT CREATE LARGER MOLECULES FROM SMALLER BUILDING BLOCKS, REQUIRED FOR GROWTH ‘BUILDING REACTIONS’

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7
Q

what are catabolic reactions?

A

REACTIONS THAT BREAK DOWN LARGE ORGANIC MOLECULES INTO SMALLER ONES, OR INTO SMALL INORGANIC MOLECULES ‘BREAKING DOWN REACTIONS’

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8
Q

what is an intracellular enzyme?

A

ENZYMES THAT ACT WITHIN CELLS

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9
Q

give an example of an intracellular enzyme and its mechanism

A

CATALASE- CATALYSES THE BREAKDOWN OF HYDROGEN PEROXIDE (H2O2) INTO WATER AND OXYGEN

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10
Q

give an example of an extracellular enzyme and its mechanism

A
  1. AMYLASE- CATALYSES THE BREAKDOWN OF STARCH INTO MALTOSE (maltase = maltose to glucose)
  2. TRYPSIN- CATALYSES THE BREAKDOWN OF PROTEINS INTO SMALLER POLYPEPTIDES (proteases = smaller polypeptides into amino acids)
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11
Q

what is an extracellular enzyme?

A

ENZYMES THAT ARE RELEASED FROM CELLS AND ACT OUTSIDE THE CELL THAT MAKES THEM
THEY DIGEST LARGE MACROMOLECULES EG. STARCH, INTO SMALLER ORGANIC MOLECULES SO THAT THEY CAN BE ABSORBED INTO CELLS (LARGE MOLECULES CAN ENTER CELLS DIRECTLY

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12
Q

what is digestion and why is it necessary?

A

IT IS THE BREAKING DOWN OF LARGE MOLECULES INTO THEIR SMALLER BUILDING BLOCKS,

  1. FOR THE NEXT REACTION EG. GLUCOSE IN RESPIRATION NOT CELLULOSE
  2. FOR TRANSPORTATION OF MOLECULES
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13
Q

example of digestion

A

POLYMERS INTO MONOMERS = TRIGLYCERIDES INTO 3 FATTY ACIDS AND GLYCEROL

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14
Q

what does a dehydrogenase enzyme do?

A

catalyses removal of H2

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15
Q

how do enzymes affect structure and function of cells and entire organisms?

A

ALL MOLECULES IN A CELL OR ORGANISM ARE PRODUCED USING AN ECR, THEREFORE THE PRESENCE, QUANTITY AND FORM OF THE MOLECULE IS DETERMINED BY THE ENZYMES PRESENT, WHICH WILL DETERMINE THE STRUCTURE OF CELLS AND ORGANISMS EG. PIGMENT MOLECULES OR STRUCTURAL PROTEINS SUCH AS COLLAGEN.
FUNCTION OF CELL ALSO DETERMINED BY ENZYMES AS THE ROLE OF THE CELL IS DETERMINED BY THE REACTIONS IT CAN PERFORM.

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16
Q

what does a dehydrogenase enzyme do?

A

CATALYSES THE REMOVAL OF H2

17
Q

how do enzymes affect structure and function of cells and entire organisms?

A

ALL MOLECULES IN A CELL OR ORGANISM ARE PRODUCED USING AN ECR, THEREFORE THE PRESENCE, QUANTITY AND FORM OF THE MOLECULE IS DETERMINED BY THE ENZYMES PRESENT, WHICH WILL DETERMINE THE STRUCTURE OF CELLS AND ORGANISMS EG. PIGMENT MOLECULES OR STRUCTURAL PROTEINS SUCH AS COLLAGEN.
FUNCTION OF CELL ALSO DETERMINED BY ENZYMES AS THE ROLE OF THE CELL IS DETERMINED BY THE REACTIONS IT CAN PERFORM.

18
Q

explain how a triglyceride is broken down

A

HYDROLASE CATALYSES TRIGLYCERIDES TO GLYCEROL AND 3 FATTY ACIDS

19
Q

explain how a triglyceride is broken down

A

HYDROLASE CATALYSES TRIGLYCERIDES TO GLYCEROL AND 3 FATTY ACIDS

20
Q

where are enzymes made?

A

RIBOSOMES

21
Q

where are enzymes usually modified?

A

IN THE GOLGI BODY

22
Q

where can a problem arise in metabolism?

A

IF THE DNA MUTATES, THE PROTEINS’ PRIMARY STRUCTURE WILL BE FAULTY, THE PROTEIN FOLDS UP INCORRECTLY, THE TERTIARY STRUCTURE FAULTY, THE ENZYME CANNOT FUNCTION CORRECTLY BECAUSE ITS THE WRONG SHAPE

23
Q

where can a problem arise in metabolism?

A

IF THE DNA MUTATES, THE PROTEINS’ PRIMARY STRUCTURE WILL BE FAULTY, THE PROTEIN FOLDS UP INCORRECTLY, THE TERTIARY STRUCTURE FAULTY, THE ENZYME CANNOT FUNCTION CORRECTLY BECAUSE ITS THE WRONG SHAPE