4: Effect of sucrose concentration on pollen tube growth Flashcards
Independent variable
sucrose concentration
Dependent variable
Length of pollen tube in given time
Variables to control
Temperature
Maturity of flower providing pollen
O2 availability
Advantages of using pollen from ONE flower
- Improves validity of results because the maturity of the flower is controlled
This means any differences in results are more likely to be a result of sucrose concentration.
Disadvantages of using pollen from ONE flower
Different results may arise if the experiment is repeated
Flowers may vary slightly in maturity
Genetic differences
Ideally the investigation would be repeated with a large number of flowers ( calculate mean)
Why do you add a moistened filter paper in the Petri dish?
The humidity in the dish stops the pollen from DRYING out
Why is there no coverslip on the slide?
To avoid ANOXIC conditions