2: Using light microscope to observe ad measure biological samples

Question 1

How do you calibrate the eye piece graticule?

Answer 1

Count the number of epu that fit into a known length on the stage micrometer.

e.g. if 100 micrometer = 4 epu,
then 1 epu = 100/4 = 25 micrometer

CALIBRATE FOR EACH MAGNIFICATION!

Question 2

What stain is added to the transverse cut sections?

Answer 2

Toluidine blue O stain

This is a metachromatic stain.

Question 3

How do you explain low powered drawings?

Answer 3

Give a simple outline by stating the tissues within your scope.

Question 4

How do you explain high powered drawings?

Answer 4

Label CELLS within your scope

(max 10 cells)