3.7 Genetics, Populations, Evolution and Ecosystems Flashcards
3.7.4 Populations in ecosystems - sampling methods
What is abundance?
The number of individuals of a species in a given space
What are the two main methods of studying abundance?
- Frequency
- The likelihood of a particular species occurring in a quadrat - Percentage cover
- Estimate of the area within the quadrat that a particular plant species covers
What are the advantages and disadvantages of frequency?
Often expressed as a percentage
Useful where species is easier to count
A quick idea of the species present
- Lacks information on the density
- No detailed information on distribution of species
- Can be quite time consuming
How do you calculate the percentage cover per quadrat?
Number of squares species covers/
Number of squares in total x100
What are the advantages and disadvantages of percentage cover?
+ Useful when a species is abundant or difficult to count
+ Data can be collected quickly
+ Gives a representation of density/ distribution
- Less useful where organisms occur in overlapping layers
- Can be less precise
- Often subjective
What are the two types of sampling?
- Random Sampling
e. g. frame quadrats and point quadrats - Systematic Sampling
e. g. Transects
What do quadrats do?
Investigate the difference between two or more sites
What three key factors need to be considered when using quadrats?
- The size of the quadrat used
- Depends on size of species being studied, size of area being studied, and whether the species are evenly distributed or clustered in small groups. If not evenly distributed, a large number of small quadrats need to be used - The number of sample quadrats to record within the study area
- Larger sample = more reliable results
- If there is a larger number of different species in sample, the greater the number of quadrats needed to produce valid results - The position of each quadrat within the study area
- Sampling MUST be random
How do you carry out random sampling (quadrats)?
Place two long tape measure at right angles along two sides of study area
Use coordinates by using random numbers from table/computer
Place a quadrat at the point of intersection
Record names and numbers of different species present
Repeat multiple times and calculate mean
Why must sampling be random?
To avoid sampling bias
Ensures data obtained is valid
Produce statistically significant results
What are the difficulties of using quadrats?
Difficult to identify and count every organism
Time consuming
May damage the habitat
Difficult to avoid introducing an element of personal bias
No indication of bias
What are point quadrats?
Horizontal bar supported by 2 legs
Pins are dropped through holes in top
Each species the pin touches is recorded
What are the advantages and disadvantages of using point quadrats?
+ Smaller organisms recorded
+ Small areas for specific detail
- Tedious
- Hard to use
- Time consuming
How could quadrats be used economically?
Construction sites (reduces environmental costs)
Optimum conditions for horticulture
Optimum conditions for agriculture
What is systematic sampling?
Studies the distribution of a species
When is systematic sampling used?
When gradual changes occur across a habitat
When transitions within communities occur
When zonation is present
What are the two types of transect?
Belt transects
Line transect
What is a line transect?
A string or tape
Organisms over which the line passes are recorded
Usually using a quadrat
What is a belt transect?
Strip or meter wide tape
Second placed parallel to first
Species between the two belts are recorded
When are transects used?
To show the trend of change in the number of two species in a given time
NOT a comparison of two areas
How do you carry out a transect?
Place transect (tape measure) down across a given area
Place quadrat down at equal intervals along the transect or at random intervals along transect
Carry out second transect in different part of the sample but still close by first sample
Calculate a mean across the 3 transects studied and display in a table
Plot a graph and describe the pattern shown
What is an alternative method to calculate population size?
Mark, release, re-capture
What is mark, release, re-capture?
A specific number of animals are caught, marked, and then released
After some time, a given number of animals are collected randomly
The number of marked animals counted
Calculate estimated population
How do you calculate estimated population?
Total no. in 1st sample x Total no. in 2nd /
Number of marked individuals recaptured x100
What assumptions must be made when using mark, release, re-capture?
- Proportion of marked to unmarked in second sample is same as proportion of marked to unmarked in whole population
- Ensures results not due to different sample sizes - Marked individuals distribute themselves evenly
- Results are not valid in clusters - The population has a definite boundary
- Ensures you are studying the entire population - Markings are nontoxic and makes species no more conspicuous
- Ensures markings do not injure or reduce survival chances - Population is constant e.g. not breeding season
- ‘New’ individuals would not be marked - Markings cannot be rubbed off during investigation
- Ensures population size in 2nd sample is not reduced
- Ensures validity
What are the advantages of using mark, release, recapture?
+ Useful for measuring abundance when organisms can move, can hide or are difficult to find and identify
What are the disadvantages of using mark, release, recapture?
- Time consuming (need to leave for a time period)
- Risk of predation changing sample size
- Disturbance of habitat
- Trauma or injury to the organism (ethics)
3.7.1 Inheritance
What is a dominant allele?
Alleles which only need one gamete to show phenotypic trait.
The allele is always expressed in the phenotype e.g., BB or Bb
What is a recessive allele?
Alleles which need both gametes to show phenotypic trait.
The allele is not always expressed in the phenotype e.g., bb
What is an allele?
A version of a gene controlling characteristics but at the same site on homologous chromosome
What is a gene?
Specific section of DNA
What are homozygous chromosomes?
Two alleles are the same e.g., BB or bb
What are heterozygous chromosomes?
Two alleles are different e.g., Bb
What is a genotype?
The genetic information coding for the phenotype e.g., BbLl
What is a phenotype?
The physical characteristic expressed as a result of the genotype e.g., eye colour
What are the rules for inheritance?
Choose a letter to represent the trait you are investigating
Choose letters that are different lower case/capital
Capital = DOMINANT
Lower case = RECESSIVE
Label parents and their phenotype (parent 1 = brown eyes)
Label gametes the parents produce and put circle around them
Draw a punnet square
Label gender of each parent
Always write dominant allele first
Work out the cross
State the phenotypes
Calculate the ratio
What is a monohybrid cross?
A cross in which the alleles of any one gene are involved
- Inheritance of a single gene
- Used to determine the dominant relationship between two alleles
- The cross begins with the parental (P1 or P) generation
Example of monohybrid cross
Step 1 : Determine the gametes from each parent.
- Parent 1 = R r
- Parent 2 = R r
Step 2 : Create punnet square
R r R RR Rr r Rr rr
Step 3 : Determine Phenotypic ratio
- 3:1
What is the basic law of genetics?
In diploid organisms, characteristics can be determined by alleles that occur in pairs
Only one of each pair of alleles can be present in a single gamete
What is pure breeding?
Pure breeding for a characteristic involves constantly breeding parent with desirable characteristic with another parent of desirable characteristic
- The organisms also become homologous
- All resulting offspring are known as first filial generation (F1)
What is a dihybrid cross?
Considers the inheritance of two characteristics at the same time
Shows how 2 characteristics, determined by 2 different genes located on different chromosomes are inherited e.g. shape and colour
Example of dihybrid cross
Seed colour = Yellow (Y, dominant) or green (y, recessive)
Seed shape = Round (R, dominant) or wrinkled (r, recessive)
Parent 1 = Round, yellow (RRYY) Parent 2 = wrinkled, green (rryy) ry ry RY RYry RYry RY RYry RYry
Phenotype of all F1 generation = round, yellow (genotype = RrYy)
Parent 1 from F1 generation = Round, yellow (RrYy) (gametes = RY, Ry, rY, ry)
Parent 2 from F1 generation = Round, yellow (RrYy) (gametes = RY, Ry, rY, ry)
RY Ry rY ry RY RRYY RRYy RrYY RrYy Ry RRYy RRyy RrYy Rryy rY RrYy RrYy rrYY rrYy ry RrYy Rryy rrYy rryy
Phenotypic ratio of F2 generation =
9 : 3 : 3 : 1
(round, yellow : round, green : wrinkled, yellow : wrinkled, green)
What is the theory of dihybrid inheritance?
The F1 generation produce 4 types of gamete.
They are able to do this as the gene for colour and gene for shape are on different chromosomes
During meiosis the chromosomes can arrange randomly at the equator
This means either shape allele can combine with either colour allele
Fertilisation is random so any of 4 gametes from one parent can mix with any of 4 from other parent
What is the law of independent assortment?
Each member of pair of alleles may combine randomly with either of another pair
What is codominance?
When both alleles are expressed in the phenotype
Neither allele is dominant nor recessive, they are both equally dominant
What are the rules for codominance?
We can’t use upper/lower case letters as this implies dominant/recessive
We use different letters instead e.g. R=Red, W=White
Letters should be superscript to the letter that represents the gene in the question e.g. Cᴿ
Example of codominance punnet square
Parent 1 = Red (CᴿCᴿ)
Parent 2 = White (CᵂCᵂ)
Cᴿ Cᴿ Cᵂ CᴿCᵂ CᴿCᵂ Cᵂ CᴿCᵂ CᴿCᵂ
Genotype of F1 generation = all CᴿCᵂ
Phenotype of F1 generation = all Pink as both colours are present
Parent 1 from F1 generation = Pink (CᴿCᵂ)
Parent 2 from F1 generation = Pink (CᴿCᵂ)
Cᴿ Cᵂ Cᴿ CᴿCᴿ CᴿCᵂ Cᵂ CᴿCᵂ CᵂCᵂ
Phenotypic ratio of F2 generation =
Pink : Red : White
2 : 1 : 1
What is the principle of multiple alleles?
Multiple alleles have more than two alternative forms of a single gene, located at the same loci of homologous chromosomes
Polygenic traits (e.g. eye colour) are determined by several genes at a different gene loci Multiple alleles are involved in determination of a single trait by codominance
How is codominance and multiple alleles related to human blood groups?
Human blood groups have 3 associated with the immunoglobulin gene (I)
Gene I dictates the presence of certain antigens on the cell surface membrane of red blood cells
Allele Antigenproduced
Iᴬ A
Iᴮ B
Iᴼ Neither
There are three alleles but only two can be present in an individual because there are only 2 homologous chromosomes, so only 2 gene loci
Iᴬ and Iᴮ are codominant
Iᴼ is recessive to both
Blood group Possible genotypes A IᴬIᴼ or IᴬIᴬ B IᴮIᴼ or IᴮIᴮ AB IᴬIᴮ O IᴼIᴼ
How does immunology link to human blood group inheritance?
Blood group A = A antigens on red blood cells with anti-B antibodies in the plasma
Blood group B = B antigens with anti-A antibodies in plasma
Blood group O = no antigens, but both anti-A and anti-B antibodies in plasma
Blood group AB = has both A and B antigens, but no antibodies
How does receiving blood from the wrong ABO group be life threatening?
If someone with group B blood is given to group A blood, their anti-A antibodies will attack group A cells
This is why group A blood must never be given to someone with group B or O
Example of human blood group punnet square
Parent 1 = Group AB (IᴬIᴮ)
Parent 2 = Group O (IᴼIᴼ)
Iᴬ Iᴮ Iᴼ IᴬIᴼ IᴮIᴼ Iᴼ IᴬIᴼ IᴮIᴼ
Phenotypic ratio =
A : B
2 : 2
Does not produce offspring of same blood type as parents
Parent 1 = Group A (IᴬIᴼ)
Parent 2 = Group B (IᴮIᴼ)
Iᴬ Iᴼ Iᴮ IᴬIᴮ IᴮIᴼ Iᴼ IᴬIᴼ IᴼIᴼ
Phenotypic ratio =
AB : A : B : O
1 : 1 : 1 : 1
By crossing A and B heterozygotes you get offspring with one of each blood types
What is the difference between chromosomes and chromatids?
Before replication, one chromosome is composed of one DNA molecule
Following replication, each chromosome is composed of two identical DNA molecules (DNA replication increases the amount of DNA but does not increase number of chromosomes)
Two identical copies, each forming one half of replicated chromosome are called chromatids
During the later stages of cell division these chromatids separate longitudinally to become individual chromosomes
What is the human karyotype?
All chromosomes of human
46 chromosomes, 23 pairs
22 of 23 pairs have homologous partners which are identical
23rd pair are sex chromosomes (X and Y)
XY = male XX = female
What is sex linkage?
Genes found on either X or Y are sex linked
What is the impact of X chromosome being longer than Y chromosome?
Some genes found on X do not have a homologous equivalent copy on Y
Recessive characteristics found on this portion will be more frequent in men because they have no homologous section on Y that could carry the dominant allele
What is haemophilia?
X-linked genetic disorder (defective gene on X)
Blood does not clot correctly – fatal if not treated.
Extremely rare in females because they have 2 X chromosomes and haemophiliac females died at puberty when menstruation begins.
Haemophilia is a recessive disorder.
The recessive allele codes for an alternative base sequence which results in faulty protein.
The healthy version of the protein allows clotting to occur.
The healthy protein is now produced by GH organisms
Punnet Square showing how haemophilia is inherited
H = clotting protein, h = non clotting protein
Parent 1 = Female Carrier (XᴴXʰ)
Parent 2 = Male Normal (XᴴY)
Xᴴ Xʰ Xᴴ XᴴXᴴ XᴴXʰ Y XᴴY XʰY
Phenotypic Ratio of F1 generation =
Healthy female : Carrier female : Healthy male : Haemophiliac male
1 : 1 : 1 : 1
What is autosomal linkage?
When 2 or more genes are carried on the same autosome
(all chromosomes that are not sex chromosomes are autosomes)
Any 2 genes found on the same chromosome are linked.
All linked genes stay together during meiosis.
This means they will pass into the gamete together meaning they will pass into offspring together.
They do not follow Mendel’s Law of Independent Assortment as each allele is not free to mix with either allele from another pair
What is epistasis?
Epistatsis describes a condition whereby one gene controls the expression of another gene.
OR
When an allele of one gene affects/masks the expression of another in the phenotype
Example of epistasis punnet square
Gene A – distribution of black pigment (melanin)
A = Banded
A = uniform colour
Gene B – coat colour by determining whether A is expresses.
B = produces melanin
B = no melanin
Agouti Mouse = Grey/Brown Banded fur with melanin (A_B_)
Albino mouse = White with no melanin at all (A_bb or aabb)
Black mouse = Uniform black hairs with no banding but has melanin (aaB_)
Parent 1 = Agouti (AABB)
Parent 2 = Albino (aabb)
AB AB ab AaBb AaBb ab AaBb AaBb
100% Agouti (AaBb) in F1 generation
Parent 1 = Agouti (AaBb)
Parent 2 = Agouti (AaBb)
AB Ab aB ab AB AABB AABb AaBB AaBb Ab AABb AAbb AaBb Aabb aB AaBB AaBb aaBB aaBb ab AaBb Aabb aaBb aabb
Phenotypic ratio of F2 generation =
Agouti : white : black
9 : 4 : 3
The expression of gene B (melanin) affects the expression of gene A (bands)
If there is no melanin, then gene A cannot be expressed (there is no pigment to form bands)
How is epistasis linked to biochemical pathways?
Some genes act in sequence by coding for specific enzymes in a pathway
Dominant alleles code for the functional form of the enzyme
Recessive homozygotes would disrupt the pathway.
The presence of one non-functional gene will affect the other as failure to express one gene will result in no pigment being made
What is a X² test/ chi squared test?
Allows us to test the significance of differences between observed and expected results
When can chi squared be used?
When the data can be put into discrete categories e.g. phenotypes, no. of organisms in an area
When there is a large sample size
When there is raw data only (not percentages/rates)
When the portion of numbers expected in each category is known
Steps for chi squared
- Formulate a null hypothesis
- There is no significant difference between ? and ? - Design a table for your data and calculations
- Carry out calculations
- Determine degrees of freedom
- (number of categories – 1) - Determine whether to reject or accept null hypothesis
- If chi value is BELOW critical value = no significant difference between observed and expected = any difference due to chance = ACCEPT null hypothesis
- If chi value is ABOVE/EQUAL TO critical value = there is significant difference between observed and expected = something other than chance is causing difference = REJECT null hypothesis
Example of chi squared question
A heterozygous long-winged fruit fly was bred with a heterozygous vestigial-winged fruit fly. They produced offspring of 145 long-winged and 55 vestigial winged
Show that the phenotypic ratio would be 3:1
Carry out chi squared test
- Draw punnet square
L l
L LL Ll
l Ll ll
Phenotypic ratio = 3:1
2. Carry out chi squared Category O E O-E (O-E)² (O-E)²/E Long-winged 145 150 -5 25 0.167 Vestigial-winged 55 50 5 25 0.5 X²= 0.667
Expected = 145 + 55 = 200
3 + 1 = 4
200/4 x 3 = 150
200/4 x 1 = 50
- Calculate degrees of freedom
2-1 = 1
Critical value = 3.84 - Conclusion
- X² value is below critical value so there is no significant difference between observed and expected. Any differences are due to chance so ACCEPT null hypothesis
3.7.2-3.7.4
What is ecology?
The study of inter-relationships between organisms and their environment