35 - Basic genetic and bioinformatic technologies

Question 1

all coding mRNA?

Answer 1

transcriptome

Question 2

all proteins and metabolites

Answer 2

proteome

metabolome

Question 3

Central dogma

Answer 3

Forms the basis of healthy activity in one cell and are abnormal in disease
- can gain diagnostic info by measuring them

Question 4

What do ncRNAs do?

Answer 4

alter translation of proteins., cellular activity and alter stability of mRNA
- range of new ways of gene regulation

Question 5

How was DNA originally sequenced

Answer 5

Sanger Sequencing

Question 6

What is Sanger Sequencing

Answer 6

• DNA sequencing to study a single gene
• involves labelling DNA with an enzyme that distends the DNA and causes it to emit colour or a radioactive band on radioactive gel
• enabled sequencing of human genome
• establish the sequence of a piece of DNA/gene

Question 7

Old Sanger sequencing method

Answer 7

Radioactive band method depends on the enzyme stopping sand a product (piece of partially made DNA with a gene added to it stopping it at a particular place when you separate the DNA)

Question 8

Newer

Answer 8

depends on fluorescent tag or colour for each different base