3.3 Organisms exchange substances with their environment Flashcards
Surface area to volume ratio
- The surface area of an organism divided by its volume
- the larger the organism, the smaller the ratio
Factors affecting gas exchange
- diffusion distance
- surface area
- concentration gradient
- temperature
Ventilation
- Inhaling and exhaling in humans
- controlled by diaphragm and antagonistic interaction of internal and external intercostal muscles
Inspiration
- External intercostal muscles contract and internal relax
- pushing ribs up and out
- diaphragm contracts and flattens
- air pressure in lungs drops below atmospheric pressure as lung volume increases
- air moves in down pressure gradient
Expiration
- External intercostal muscles relax and internal contract
- pulling ribs down and in
- diaphragm relaxes and domes
- air pressure in lungs increases above atmospheric pressure as lung volume decreases
- air forced out down pressure gradient
Passage of gas exchange
- Mouth/nose → trachea → bronchi → bronchioles → alveoli
- crosses alveolar epithelium into capillary endothelium
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Alveoli structure
- Tiny air sacs
- highly abundant in each lung – 300 million
- surrounded by the capillary network
- epithelium 1 cell thick
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Why large organisms need specialised exchange surface?
- They have a small surface area to volume ratio
- higher metabolic rate – demands efficient gas exchange
- specialised organs e.g. lungs/gills designed for exchange
Fish gill anatomy
- Fish gills are stacks of gill filaments
- each filament is covered with gill lamellae at right angles
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How fish gas exchange surface provides large surface area?
- Many gill filaments covered in many gill lamellae are positioned at right angles
- creates a large surface area for efficient diffusion
Countercurrent flow
- When water flows over gills in opposite direction to flow of blood in capillaries
- equilibrium not reached
- diffusion gradient maintained across entire length of gill lamellae
Name three structures in tracheal system
- Involves trachea, tracheoles, spiracles
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How tracheal system provides large surface area?
- Highly branched tracheoles
- large number of tracheoles
- filled in ends of tracheoles moves into tissues during exercise:
- so larger surface area for gas exchange
Fluid-filled tracheole ends
- Adaptation to increase movement of gases
- when insect flies and muscles respire anaerobically – lactate produced
- water potential of cells lowered, so water moves from tracholes to cells by osmosis
- gases diffuse faster in air
How do insects limit water loss?
- Small surface area to volume ratio
- waterproof exoskeleton
- spiracles can open and close to reduce water loss
- thick waxy cuticle – increases diffusion distance so less evaporation
Dicotyledonous plants leaf tissues
- Key structures involved are mesophyll layers
- (palisade and spongy mesophyll)
- stomata created by guard cells
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Gas exchange in plants
- Palisade mesophyll is site of photosynthesis
- oxygen produced and carbon dioxide used creates a concentration gradient
- oxygen diffuses through air space in spongy mesophyll and diffuse out stomata
Role of guard cells
- swell – open stomata
- shrink – closed stomata
- at night they shrink, reducing water loss by evaporation
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Xerophytic plants
- Plants adapted to survive in dry environments with limited water (e.g. marram grass/cacti)
- structural features for efficient gas exchange but limiting water loss
Adaptations of xerophyte
- Adaptations to trap moisture to increase humidity → lowers water potential inside plant so less water lost via osmosis:
- sunken stomata
- curled leaves
- hairs
– - thick cuticle reduces loss by evaporation
- longer root network
Digestion
- Process where large insoluble biological molecules are hydrolysed into smaller soluble molecules
- so they can be absorbed across cell membranes
Locations of carbohydrate digestion
- Mouth → duodenum → ileum
Locations of protein digestion
- Stomach → duodenum → ileum
Endopeptidases
- Break peptide bonds between amino acids in the middle of the chain
- creates more ends for exopeptidases for efficient hydrolysis
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Exopeptidases
- Break peptide bonds between amino acids at the ends of polymer chain
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Membrane-bound dipeptidases
- Break peptide bond between two amino acids
Digestion of lipids
- Digestion by lipase (chemical)
- emulsified by bile salts (physical)
- lipase produced in pancreas
- bile salts produced in liver and stored in gall bladder
Lipase
- Produced in pancreas
- Breaks ester bonds in triglycerides to form:
- monoglycerides
- glycerol
- fatty acids
Role of bile salts
- Emulsify lipids to form tiny droplets and micelles
- increases surface area for lipase action – faster hydrolysis
Micelles
- Water soluble vesicles formed from fatty acids, glycerol, monoglycerides and bile salts
Lipid absorption
- Micelles deliver fatty acids, glycerol and monoglycerides to epithelial cells of ileum for absorption
- cross via simple diffusion as lipid-soluble and non-polar
Lipid modification
- Smooth ER reforms monoglycerides/fatty acids into tryglycerides
- golgi apparatus combines tryglycerides with proteins to form vesicles called chylomicrons
How lipids enter blood after modification?
- Chylomicrons move out of cell via exocytosis and enter lacteal
- lymphatic vessels carry chylomicrons and deposit them in bloodstream
How are glucose and amino acids absorbed?
- Via co-transport in the ileum
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Haemoglobin (Hb)
- Quaternary structure protein:
- 2 alpha chains
- 2 beta chains
- with 4 associated haem groups in each chain containing Fe2+
– - transports oxygen
Affinity of haemoglobin
- The ability of haemoglobin to attract/bind to oxygen
Saturation of haemoglobin
- When haemoglobin is holding the maximum amount of oxygen it can hold
Loading/unloading of haemoglobin
- Binding/detachment of oxygen to haemoglobin
- also known as association and disassociation
Oxyhaemoglobin dissociation curve
- oxygen is loaded in regions with high partial pressures (alveoli)
- unloaded in regions of low partial pressure (respiring tissue)
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Oxyhaemoglobin dissociation curve shifting left
- Hb would have a higher affinity for oxygen
- load more at the same partial pressure
- becomes more saturated
- adaptation in low-oxygen environments
- e.g. llamas/in foetuses
Cooperative binding
- Hb’s affinity for oxygen increases as more oxygen molecules are associated with it
- when one binds, Hb changes shape meaning others bind more easily
- explaining S shape of curve
How carbon dioxide affects haemoglobin?
- When carbon dioxide dissolves in liquid, carbonic acid forms
- decreases pH causing Hb to change shape
- affinity decreases at respiring tissues
- more oxygen is unloaded
Bohr effect
- High carbon dioxide partial pressure
- causes oxyhaemoglobin curve to shift to the right
Oxyhaemoglobin dissociation curve shifting right
- Hb has lower affinity for oxygen
- unloads more at the same partial pressures
- less saturated
- present in animals with faster metabolisms that need more oxygen for respiration
- e.g. birds/rodents
Closed circulatory system
- Blood remains within blood vessels
Name different types of blood vessels
- Arteries, arterioles, capillaries, venules and veins
Structure of arteries
- Thick muscular layer
- thick elastic layer
- thick outer layer
- small lumen
- no valves
Capillary endothelium
- Extremely thin
- one cell thick
- contains small gaps for small molecules to pass through (e.g. glucose, oxygen)
Capillaries
- Form capillary beds
- narrow diameter (1 cell thick) to slow blood flow
- red blood cells squashed against walls shortens diffusion pathway
- small gaps for liquid/small molecules to be forced out
Arterioles
- Branch off arteries
- thickest muscle layer to restrict blood flow
- thinner elastic layer and outer layer than arteries as pressure lower
Tissue fluid
- Liquid bathing all cells
- contains water, glucose, amino acids, fatty acids, ions and oxygen
- enables delivery of useful molecules to cells and removal of waste
Tissue fluid formation
- At arteriole end, the smaller diameter results in high hydrostatic pressure
- small molecules forced out (ultrafiltration)
- red blood cells/large proteins too big to fit through capillary gaps so remain
Reabsorption of tissue fluid
- Large molecules remaining in capillary lower its water potential
- towards venule end there is lower hydrostatic pressure due to loss of liquid
- water reabsorbed back into capillaries by osmosis
Role of the lymph in tissue fluid reabsorption
- Not all liquid will be reabsorbed by osmosis as equilibrium will be reached
- excess tissue fluid (lymph) is absorbed into lymphatic system and drains back into bloodstream and deposited near heart
Cardiac muscle
- Walls of heart having thick muscular layer
- unique because it is:
- myogenic – can contract and relax without nervous or hormonal stimulation
- never fatigues so long as adequate oxygen supply
Coronary arteries
- Blood vessels supplying cardiac muscle with oxygenated blood
- branch off from aorta
- if blocked, cardiac muscle will not be able to respire, leading to myocardial infarction (heart attack)
Structure of the heart
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Adaptation of left ventricle
- Has a thick muscular wall in comparison to right ventricle
- enables larger contractions of muscle to create higher pressure
- ensures blood reaches all body cells
Veins connect to the heart
- Vena cava – carries deoxygenated blood from body to right atrium
- Pulmonary vein – carries oxygenated blood from lungs to left atrium
Arteries connected to the heart
- Pulmonary artery – carries deoxygenated blood from right ventricle to lungs
- Aorta – carries oxygenated blood from left ventricle to rest of the body
Valves within the heart
- Ensure unidirectional blood flow
- semilunar valves are located in aorta and pulmonary artery near the ventricles
- atrioventricular valves between atria and ventricles
Opening and closing of valves
- Valves open if the pressure is higher behind them compared to in front of them.
- AV valves open when pressure in atria > pressure in ventricles
- SL valves open when pressure in ventricles > pressure in arteries
The Septum
- Muscle that runs down the middle of the heart
- separates oxygenated and deoxygenated blood
- maintains high concentration of oxygen in oxygenated blood
- maintaining concentration gradient to enable diffusion to respiring cells
Cardiac output
- Volume of blood which leaves one ventricle in one minute.
- heart rate = beats per minute
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Stroke volume
- Volume of blood that leaves the heart each beat
- measured in dm^3
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Cardiac cycle
- Consists of diastole, atrial systole and ventricular systole
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Diastole
- Atria and ventricular muscles are relaxed
- when blood enters atria via vena cava and pulmonary vein
- increasing pressure in atria
Atrial systole
- Atria muscular walls contract, increasing pressure further.
- pressure atria > pressure ventricles
- atrioventricular valves open and blood flows into ventricles
- ventricular muscle relaxed
Ventricular systole
- After a short delay (so ventricles fill), ventricular muscular walls contract
- pressure ventricle > atria pressure and artery pressure
- atrioventricular valves close and semi-lunar valves open
- blood pushed into artery
Transpiration
- Loss of water vapour from stomata by evaporation
- affected by:
- light intensity
- temperature
- humidity
- wind
– - can be measured in a lab using a potometer
How light intensity affects transpiration?
- As light intensity increases, rate of transpiration increases
- more light means more stomata open
- larger surface area for evaporation
How temperature affects transpiration?
- As temperature increases, rate of transpiration increases
- the more heat there is, the more kinetic energy molecules have
- faster moving molecules increases evaporation
How humidity affects transpiration?
- As humidity increases, transpiration decreases
- the more water vapour in the air, the greater the water potential outside the leaf
- reduces water potential gradient and evaporation
How wind affects transpiration?
- As wind increases, rate of transpiration increases
- the more air movement, the more humid areas are blown away
- maintains water potential gradient, increasing evaporation
Cohesion in plant transport
- Because of the dipolar nature of water, hydrogen bonds can form – cohesion
- water can travel up xylem as a continuous column
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Adhesion in plant transport
- Water can stick to other molecules (xylem walls) by forming H-bonds
- helps hold water column up against gravity
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Root pressure in plant transport
- As water moves into roots by osmosis, the volume of liquid inside the root increases
- ∴ the pressure inside the root increases
- this forces water upwards
Cohesion-tension theory
- As water evaporates out the stomata, this lowers pressure
- water is pulled up xylem (due to negative pressure)
- cohesive water molecules creates a column of water
- water molecules adhere to walls of xylem pulling it upwards
- this column creates tension, pulling xylem inwards
Translocation
- Occurs in phloem
- explained by mass flow hypothesis
- transport of organic substances through plant
Sieve tube elements
- Living cells
- contain no nucleus
- few organelles
- this makes cell hollow
- allowing reduced resistance to flow of sugars
Companion cell
- Provide ATP required for active transport of organic substances
- contains many mitochondria
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Mass flow hypothesis
- Organic substances, sucrose, move in solution from leaves (after photosynthesis) to respiring cells
- source → sink direction
How is pressure generated for translocation?
- Photosynthesising cells produce glucose which diffuses into companion cell
- companion cell actively transports glucose into phloem
- this lowers water potential of phloem so water moves in from xylem via osmosis
- hydrostatic pressure gradient generated
What happens to sucrose after translocation?
- Used in respiration at the sink
- stored as insoluble starch
Investigating translocation
- Can be investigated using tracer and ringing experiments
- proves phloem transports sugars not xylem
Tracing
- Involves radioactively labelling carbon – used in photosynthesis
- create sugars with this carbon
- thin slices from stems are cut and placed on X-ray film which turns black when exposed to radioactive material
- stems will turn black as that is where phloem are
Ringing experiments
- Ring of bark (and phloem) is peeled and removed off a trunk
- consequently, the trunk swells above the removed section
- analysis will show it contains sugar
- when phloem removed, sugar cannot be transported
How do small organisms exchange gases?
- Simple diffusion
- across their surface
Why don’t small organisms need breathing systems?
- They have a large surface area to volume ratio
- no cells far from the surface
How alveoli structure relates to its function?
- Round shape & large number in – large surface area for gas exchange (diffusion)
- epithelial cells are flat and very thin to minimise diffusion distance
- capillary network maintains concentration gradient
How fish gas exchange surface provides a short diffusion distance?
- Thin lamellae epithelium means short distance between water and blood
- capillary network in every lamella
How fish gas exchange surface maintains diffusion gradient?
- Counter-current flow mechanism
- circulation replaces blood saturated with oxygen
- Ventilation replaces water with oxygen removed
Name of gas exchange system in terrestrial insects
- Tracheal system
Describe structure of spiracles
- Round, valve-like openings
- running along the length of the abdomen
Describe trachea & tracheoles structure
- Network of internal tubes
- have rings of cartilage adding strength and keeping them open
- trachea branch into smaller tubes – tracheoles
- tracheoles extend through all tissues delivering oxygen
How tracheal system provides short diffusion distance?
- Tracheoles have thin walls so short diffusion distance to cells
How tracheal system maintains concentration gradient?
- Body can be moved by muscles to move air –ventilation
- Use of oxygen in respiration and production of CO2 sets up steep concentration gradients
Amylase
- Produced in pancreas & salivary gland
- hydrolyses starch into maltose
Membrane-bound disaccharidases
- Maltase/sucrase/lactase
- hydrolyse disaccharides into monosaccharides
Enzymes involved in protein digestion
- endopeptidases
- exopeptidases
- membrane-bound dipeptidases
Products of protein digestion
- Large polymer proteins are hydrolysed to amino acids
Double circulatory system
- Blood passes through heart twice
- pulmonary circuit delivers blood to/from lungs
- systemic circuit delivers blood to the rest of the body
Coronary arteries
- Supply cardiac muscle with oxygenated blood
- for continued respiration and energy production for contraction
Blood vessels entering/exiting the kidney
- Renal artery carries oxygenated blood to kidney
- renal vein carries deoxygenated blood to heart
Blood vessels entering/exiting the lung
- Pulmonary artery carries deoxygenated blood to lung
- pulmonary vein carries oxygenated blood to heart
Blood vessels entering/exiting the heart
- Vena cava carries deoxygenated blood to heart (right atrium)
- aorta carries oxygenated blood to body
- pulmonary artery – carries blood from the heart to the lungs
- pulmonary vein – carries blood from the lungs into the heart
Describe then structure of veins
- Thin muscular layer
- thin elastic layer
- thin walls
- valves
Explain role of elastic layer in arteries
- Thick elastic layer
- to help maintain blood pressure
- by stretching and recoiling
Describe the elastic layer in veins
- Thin elastic layer as pressure lower
- cannot control the flow of blood
Explain the role of valves in veins
- Due to low pressure in veins
- skeletal muscle usually used to flatten walls of veins for blood flow
- valves prevent the backflow of blood
- unidirectional flow
What causes the AV valves to open?
- Higher pressure in the atria than in the ventricles
What causes the semi-lunar valves to open?
- Higher pressure in the ventricles than in the arteries