3.3 microscope measurements and calculations

Question 1

method for calibration

Answer 1

1. align eyepiece graticule and stage micrometer
2. count graticule divisions against a set number of micrometer divisions
3. calculate size of each graticule unit

Question 2

what is usually the length of a sub-division on the stage micrometer

Answer 2

10 micrometers

Question 3

what is the eyepiece graticule

Answer 3

glass disc scale placed on eyepiece lens which is measured in arbitary units

Question 4

what is the stage micrometer

Answer 4

scale fitted onto coverslip where divisions are known (usually 10micrometre per each sub-division)

Question 5

magnification equation

Answer 5

image = actual x magnification