3.2 microscopes

Question 1

light microscope info

Answer 1

-cheap and easy to use
-light source is passed through specimen and image is reflected by glass lenses
-2D image
-resolution of 200nm
-magnifcation of x2000

Question 2

transmission electron microscope info

Answer 2

-beam of electron passed through specimen from below, parts of specimen absorb electrons to appear dark
-flat 2D image formed
-has 0.1nm resolving power (better than SEM)

Question 3

transmission electron microscope limitations

Answer 3

-must be a vaccuum
-specimen must be very thin
-may contain artefacts (not accurate image)
-complex staining process required

Question 4

scanning electron microscope info

Answer 4

-beam of electron from above onto specimen
-electron are scattered and pattern allows for a 3D image to be made
-lower resolving power of 20nm

Question 5

scanning electron microscope limitations

Answer 5

-must be a vaccuum
-may contain artefacts (not accurate image)
-complex staining process required