3 Transcription

Question 0

Second strand

Answer 0

(-) DNA template strand, RNA polymerase uses this as a template

Question 1

Top strand

Answer 1

(+) DNA coding strand, RNA produced has same sequence and polarity as it

Question 2

DNA

Answer 2

T

Question 3

RNA

Answer 3

U

Question 4

RNA polymerase

Answer 4

Reads the template strand

Question 5

Transcription

Answer 5

Making a polymer of ribonucleic acid (RNA) that has a sequence complementary and antiparallel to a strand of template DNA

Question 6

Sigma factor

Answer 6

Important for RNA polymerase in bacteria and prokaryotes -binds to an element in the promoter with sequence TATAAT, the tayra box - when it binds to the promoter, it recruits DNA polymerase - released when transcription starts

Question 7

TATA box

Answer 7

10 base pairs upstream of the promoter in eukaryotes only

Question 8

Transcription Eukaryotes

Answer 8

Tata box is about 25base pairs upstream of transcription start site - bound by tata binding protein (tbp) - many coactivators and basal transcription factors also help start transcription

Question 9

Lac operon

Answer 9

Allows switch between using glucose as fuel and using lactose as fuel- glucose is preferred bc it can generate ATP more efficiently