3. Connective tissue Flashcards
what are derived from haematopoetic stem cells?
monocytes macrophages lymphocytes mast cells blood cells and others
which cells produce elastin
fibroblasts
name a ‘visibile’ extracellular fibre
Reticulin
type V collagen is prevalent in which tissue type
placenta
what are derived from undifferentiated mesenchymal cells?
fibroblast, fat cells, most cartilage and bone cells
constituents of connective tissue
Cells – fibroblasts/cytes, adipose cells
Visible fibres – collagen, elastic, reticulum
Ground substance (hydrophilic jelly) – proteoglycans, glycosaminoglycans (GAGS), laminin, fibronectin etc (invisible fibres
types of connective tissue
- Fibrous – loose/ dense
- Hard – cartilage/ bone
- Fatty – white/brown
collagen
- Tropocollagen – triple helix of peptides
- Fibroblasts – secrete tropocollagen subunits
- Fibres assembled extra-cellularly
- More than 20 types
types of collagen
- Type I – skin
- Type II – cartilage
- Type III – liver, bone marrow, spleen (reticulin)
- Type IV – basement membranes
- Type V – placenta
collagen fibres
- Extracellular fibres that stain pink with H&E
- Variable thickness and length, often run in bundles
- Sometimes confused for muscle fibres that also stain pink (but are intra-cellular)
loose connective tissue
Comprises:
• Widely spaced thin collagen fibres
• Fibroblasts/fibrocytes
• Unstained ground substance
dense connective tissue
Comprises: • Closely spaced thick collagen fibres • Fibroblasts/fibrocytes • Unstained ground substance • May be irregular (top) or regular (bottom)
reticulum
- Type II collagen
- Fibrillar collagen
- Forms a supporting scaffold in many organs: bone marrow, liver, kidney, lymph node, spleen
- Not visible on H+E – need a silver stain
elastin
- Elastic tissue contains fine fibres and sheets of elastin
- Elastin fibres may be branched
- Produced by fibroblasts
- Stain pink on H+E
- Easily confused with smooth muscle cells
white adipose (fatty) tissue
- Large cells with single fat globule in each cell
* Usually appear empty (white) in conventional slides as fat is extracted during processing