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[MT 6318] Hematology Laboratory > [2S] UNIT 8 RBC Counting > Flashcards

[2S] UNIT 8 RBC Counting Flashcards

1
Q

The number of erythrocytes contained in 1 liter of blood is called?

A

Erythrocyte Number Concentration

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2
Q

T/F: The results of an RBC count can be used to help diagnose blood-related conditions, such as anemia, polycythemia and others

A

T

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2
Q

What tube should be used when collecting blood for RBC count?

A

EDTA - Anticoagulated blood

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3
Q

In traditional units, Erythrocyte Number Concentration is expressed as the?

A

number of cells per cubic millimeter

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4
Q

In traditional units, Erythrocyte Number Concentration is expressed as the number of cells per cubic millimeter and is called the?

A

Erythrocyte or Red Blood Cell Count

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5
Q

T/F: The RBC pipette has a wider bore

A

F; smaller

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6
Q

Aspirate up to?

A

0.5-1.0

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7
Q

Tube that retains cell morphology

A

EDTA/Lavender Top

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8
Q

5 tertiary squares (R squares) of the central secondary square

A

Hemacytometer

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9
Q

CHARACTERISTICS OF A GOOD RBC DILUTING FLUID

T/F: Must be a hypertonic solution

A

F; isotonic (balanced solute & solvent)

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10
Q

CHARACTERISTICS OF A GOOD RBC DILUTING FLUID

T/F: Must have a high specific gravity

A

T

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11
Q

CHARACTERISTICS OF A GOOD RBC DILUTING FLUID

T/F: Must be easy to prepare and cheap

A

T

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12
Q

CHARACTERISTICS OF A GOOD RBC DILUTING FLUID

T/F: Must be a good preservative

A

T

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13
Q

CHARACTERISTICS OF A GOOD RBC DILUTING FLUID

T/F: Must not have buffer action

A

F; must have buffer action

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14
Q

CHARACTERISTICS OF A GOOD RBC DILUTING FLUID

T/F: Must not initiate the growth of molds

A

T

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14
Q

COMPOSITION
- 40% solution of formaldehyde: 10.0 mL
- 3% W/V trisodium citrate: 990.0 mL

A

Dacie’s / Formol Citrate

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15
Q

Best RBC diluting fluid since cell shape is not altered

A

Dacie’s / Formol Citrate

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16
Q

Can be stored for long periods of time without growing fungi or molds

A

Dacie’s / Formol Citrate

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17
Q

COMPOSITION

○ Mercuric chloride, cp: 0.50 gm
○ Sodium chloride, cp: 1.00 gm
○ Sodium sulfate, cp crystalline: 5.00 gm
○ or anhydrous: 2.65 gm
○ Distilled water: 200.00 m

A

Hayem’s (Me SCared ASs Duck)

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17
Q

Allows growth of yeast

A

Hayem’s

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18
Q

Produces clumping of cells

A

Hayem’s

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19
Q

Can stand for long period

A

Hayem’s

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20
Q

No corrosive effect

A

Hayem’s

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21
Q

Prevents Rouleaux formation

A

Gower’s

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21
Q

COMPOSITION

○ Glacial acetic acid: 16.65 gm
○ Sodium sulfate, cp crystalline: 6.25 gm
○ Distilled water: 100.00 ml

A

Gower’s

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22
Q

GOWER’S

Stack of coins

A

Rouleaux formation

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22
Q

Precipitates protein in hemoglobinemia and hyperglobulinemia

A

Gower’s

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23
Q

GOWER’S

Usually observed in infection (multiple myeloma, connective tissue disorders)

A

Rouleaux formation

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24
Q

COMPOSITION

○ Methyl violet: 0.025 gm
○ Sodium Chloride: 1.00 gm
○ Sodium sulfate: 8.00 gm
○ Glycerine: 30.00 gm
○ Distilled water: 200.00 ml

A

Toisson’s (MeV SCared SiS GanDa)

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25
Q

Stains WBC to aid in the differentiation of WBC from RBCs.

A

Toisson’s

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26
Q

Beginners are recommended to use this because WBC are easily identified.

A

Toisson’s

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27
Q

Supports growth of fungi

A

Toisson’s

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28
Q

Has high specific gravity

A

Toisson’s

29
Q

Can stain WBC

A

Toisson’s

30
Q

The use of distilling fluid should also be filtered from time to time to prevent the growth of fungi

A

Toisson’s

30
Q

COMPOSITION

○ Sodium chloride: 1.00 gm
○ Sodium merthiolate, 1:1000 solution: 2.00 ml
○ Sodium sulfate, crystalline: 5.00 gm
○ Glycerine: 20.00 gm
○ Distilled water: 200.00 ml

A

Bethell’s (SCared So Much SiS GanDa)

31
Q

Other name for normal saline solution

A

Physiologic Saline Solution

32
Q

Used in emergency cases and excessive rouleaux formation and auto agglutinated cells

A

Normal Saline Solution / Physiologic Saline Solution

33
Q

It is stable and serves as a preservative

A

Normal Saline Solution / Physiologic Saline Solution

34
Q

COMPOSITION
○ Sodium Citrate
○ Distilled Water

A

3.8% Sodium Citrate

34
Q

COMPOSITION
○ Sodium chloride
○ Distilled water

A

Normal Saline Solution / Physiologic Saline Solution

35
Q

PROCEDURE

T/F: The first step is to mix your EDTA tube or blood sample before aspirating.

A

T

35
Q

PROCEDURE

T/F: After aspirating, do not wipe off the excess blood at the tip of the pipette

A

F; Wipe off excess blood at the tip of the pipette using a clean piece of tissue

36
Q

PROCEDURE

T/F: Draw the diluting fluid up to the 101 mark

A

T

37
Q

PROCEDURE

T/F: Mix the contents of the pipette while in vertical position

A

F; horizontal

38
Q

PROCEDURE

T/F: When mixing the contents of the pipette, secure the pipette by using your thumb and middle finger to make sure the contents are in homogenous solution by mixing it.

A

T

38
Q

Dilution factors

A

100 or 200

39
Q

PROCEDURE

T/F: Place the thick coverslip. Before charging we must remember to place the thick coverslip and to make sure that the coverslip and counting chamber are free from dirt, thumb marks, tissue strands or any contamination.

A

T

39
Q

PROCEDURE

T/F: Before charging, do not reshake the pipette.

A

F; Before charging, make sure to reshake the pipette.

40
Q

PROCEDURE

T/F: We discard the first 5-6 drops because the mixture or solution present in the capillary stem, which is the first few drops of the diluted blood, are cell free.

A

T

40
Q

CHARGE

Angle of the pipette while charging

A

30-35 degrees

41
Q

CHARGE

Fill the counting chamber by capillary action or the?

A

Hanging Drop Technique

42
Q

CHARGE

Let the counting chamber stand for how many minutes

A

5-10 mins

43
Q

SOURCES OF ERROR IN RBC COUNTING

Did not discard the first few drops from the pipette

A

Operator’s Technical Errors

44
Q

SOURCES OF ERROR IN RBC COUNTING

Incorrect aspiration/dilution

A

Operator’s Technical Errors

44
Q

3 Sources of Error in RBC Counting (Manual)

A

ERRORS DUE TO:
- Materials / Equipment
- Nature of Sample
- Operator’s Technical Errors

45
Q

SOURCES OF ERROR IN RBC COUNTING

Overcharging / Undercharginh

A

Operator’s Technical Errors

46
Q

SOURCES OF ERROR IN RBC COUNTING

Presence of air bubbles upon charging

A

Operator’s Technical Errors

47
Q

SOURCES OF ERROR IN RBC COUNTING

Incorrect counting (did not exclude bottom/right)

A

Operator’s Technical Errors

48
Q

SOURCES OF ERROR IN RBC COUNTING

After charging, immediately stage the counting chamber in the microscope and start the count

A

Operator’s Technical Errors

49
Q

SOURCES OF ERROR IN RBC COUNTING

Presence of dirt or debris in coverslip/counting chamber

A

Errors Due To Materials / Equipment

50
Q

SOURCES OF ERROR IN RBC COUNTING

Use of old or unfiltered diluting fluid (may lead to growth of fungi/presence of yeast cells)

A

Errors Due To Materials / Equipment

51
Q

SOURCES OF ERROR IN RBC COUNTING

RBC pipette used was not clean and dry (red bead should be moving freely)

A

Errors Due To Materials / Equipment

52
Q

2 Variations in Technique

A

RBC is markedly increased & decreased

53
Q

2 VARIATIONS IN TECHNIQUE

Increase the volume of aspirated blood. Compute for dilution accordingly

A

RBC is markedly decreased

53
Q

2 VARIATIONS IN TECHNIQUE

Decrease the volume of aspirated blood. Compute for dilution accordingly

A

RBC is markedly increased

54
Q

2 VARIATIONS IN TECHNIQUE

Use standard blood volume but increase the number of tertiary squares to count. Compute for the revised area factor accordingly

A

RBC is markedly decreased

55
Q

CORRELATION

5 Pathologic Variation

A

PAHDS

  1. Polycythemia
  2. Anemia/Oligocythemia
  3. Hemorrhage
  4. Drugs
  5. Secondary to other disorders/disease
56
Q

CORRELATION: PHYSIOLOGIC VARIATION

T/F: Men have higher counts than women

A

T

57
Q

CORRELATION: PHYSIOLOGIC VARIATION

T/F: Adults have higher counts than newborns

A

F; Newborns have higher counts than adults

58
Q

CORRELATION: PHYSIOLOGIC VARIATION

T/F: Dehydration and strenuous activities may result to decreased counts

A

F; increased

59
Q

● Principle: Solution Turbidity
● The more turbid the solution, the more cells are present in the solution

A

Photoelectric Method

60
Q

CORRELATION: PHYSIOLOGIC VARIATION

T/F: Decreased counts in individuals living at higher altitudes

A

F; increased

61
Q

3 other methods for RBC counting

A

Unopette System
Photolelectric Method
Automated Analyzers

62
Q

Principle: Electronic Impedance/ Flow Cytometer/ Coulter principle

A

Automated Analyzers

63
Q

Reference range for males

A

4.5-6.0 M/cumm

64
Q

Reference range for females

A

4.0-5.5 M/cumm

65
Q

Reference range for late pregnancy

A

3.0-5.0 M/cumm

66
Q

Reference range at birth

A

7.0 M/cumm

67
Q

Conversion of CU to SI

A

Conventional x 0.000001

68
Q

S.I. unit for RBC count

A

x10^12/L

[MT 6318] Hematology Laboratory (3 decks)

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