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[MT 6320] Clinical Bacteriology Laboratory > [2S] UNIT 6 Motility Testing (Exp 7) > Flashcards

[2S] UNIT 6 Motility Testing (Exp 7) Flashcards

1
Q

Non-staining method for demonstration of presence of flagella

A

Hanging Drop Method

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2
Q

long, proteinaceous filaments for locomotion

A

Flagella

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3
Q

Considered as a virulence factor

A

Flagella

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4
Q

for serotyping

A

Flagellar antigens

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5
Q

Presence or absence of flagella is interpreted as

A

motile or non-motile

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6
Q

Specimen used for HDM

A

Broth cultures of known motile or non-motile bacteria

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7
Q

HDM PROCEDURE

Using clean toothpick / applicator stick surround the edge of the well with thin layer of _____

A

petroleum jelly

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8
Q

HDM PROCEDURE

Place the concavity slide on a flat surface for __ minutes

A

1-2

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9
Q

HDM PROCEDURE

Examine under

A

LPO then HPO

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10
Q

HDM PROCEDURE

Color of bacterial cells

A

Translucent with slight greenish color

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11
Q

HDM PROCEDURE

True motility movement

A

bacterial cells exhibit directional purposeful movement

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12
Q

HDM PROCEDURE

Non-motile bacteria movement

A

Brownian movement

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13
Q

HDM PROCEDURE

random jiggling or shaking due to bombardment of water molecules

A

Brownian movement

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14
Q

TEST FOR MOTILITY : USE OF SIM

Specimen used

A

Broth cultures of known motile or non-motile bacteria

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15
Q

TEST FOR MOTILITY : USE OF SIM

Medium used

A

Sulfide Indole Medium

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16
Q

SIM PROCEDURE

Obtain a prepared semi-solid medium and allow it to reach ________

A

room temperature

17
Q

SIM PROCEDURE

Inoculate the culture by making a single stab down the center of the tube to about ____ the depth of the medium

A

three-fourths

17
Q

SIM PROCEDURE

Growth Pattern: bacterial growth is away from the site of inoculation

A

Motile

18
Q

SIM PROCEDURE

Incubate the medium for?

A

18 - 24 hrs at 35 - 37°C

19
Q

SIM PROCEDURE

Growth Pattern: bacterial growth is limited to the site
of inoculum

A

Non-motile

20
Q

SIM PROCEDURE

Appearance of motile

A

diffuse or hazy

21
Q

SIM PROCEDURE

Appearance of non-motile

A

Surrounding medium is clear

[MT 6320] Clinical Bacteriology Laboratory (10 decks)

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