[2S] UNIT 6 Motility Testing (Exp 7)

Question 1

Non-staining method for demonstration of presence of flagella

Answer 1

Hanging Drop Method

Question 2

long, proteinaceous filaments for locomotion

Answer 2

Flagella

Question 3

Considered as a virulence factor

Answer 3

Flagella

Question 4

for serotyping

Answer 4

Flagellar antigens

Question 5

Presence or absence of flagella is interpreted as

Answer 5

motile or non-motile

Question 6

Specimen used for HDM

Answer 6

Broth cultures of known motile or non-motile bacteria

Question 7

HDM PROCEDURE

Using clean toothpick / applicator stick surround the edge of the well with thin layer of _____

Answer 7

petroleum jelly

Question 8

HDM PROCEDURE

Place the concavity slide on a flat surface for __ minutes

Answer 8

1-2

Question 9

HDM PROCEDURE

Examine under

Answer 9

LPO then HPO

Question 10

HDM PROCEDURE

Color of bacterial cells

Answer 10

Translucent with slight greenish color

Question 11

HDM PROCEDURE

True motility movement

Answer 11

bacterial cells exhibit directional purposeful movement

Question 12

HDM PROCEDURE

Non-motile bacteria movement

Answer 12

Brownian movement

Question 13

HDM PROCEDURE

random jiggling or shaking due to bombardment of water molecules

Answer 13

Brownian movement

Question 14

TEST FOR MOTILITY : USE OF SIM

Specimen used

Answer 14

Broth cultures of known motile or non-motile bacteria

Question 15

TEST FOR MOTILITY : USE OF SIM

Medium used

Answer 15

Sulfide Indole Medium

Question 16

SIM PROCEDURE

Obtain a prepared semi-solid medium and allow it to reach ________

Answer 16

room temperature

Question 17

SIM PROCEDURE

Inoculate the culture by making a single stab down the center of the tube to about ____ the depth of the medium

Answer 17

three-fourths

Question 17

SIM PROCEDURE

Growth Pattern: bacterial growth is away from the site of inoculation

Answer 17

Motile

Question 18

SIM PROCEDURE

Incubate the medium for?

Answer 18

18 - 24 hrs at 35 - 37°C

Question 19

SIM PROCEDURE

Growth Pattern: bacterial growth is limited to the site
of inoculum

Answer 19

Non-motile

Question 20

SIM PROCEDURE

Appearance of motile

Answer 20

diffuse or hazy

Question 21

SIM PROCEDURE

Appearance of non-motile

Answer 21

Surrounding medium is clear