2c Polymerase Chain Reaction

Question 1

Polymerase Chain Reaction (PCR)

What is the first stage

Answer 1

DNA is heated (92-98°c) to separate the strands

Question 2

What is the second stage of PCR?

Answer 2

Cooling (50-65°c) allows primers to bind to target sequences

Question 3

What is the third stage of PCR?

Hint of needed
Heated to ______ to allow ______ ______ _____ polymerase to add _____ to the pr____ and repl_____ region of ______

Answer 3

Heated 70-80°c to allow heat tolerant DNA polymerase add nucleotides to primers and replicate region of DNA

Question 4

What can PCR be used for ?

Answer 4

Solving crimes

Question 5

Medical applications of PCR

Fill in blanks

Allows DNA to be _____ for the ____/____ of a specific ______ to a _____ disease

Answer 5

Screened
Presence/absence
Characteristic
Genetic

Question 6

What is one thing PCR allows experts to do?

Answer 6

To confirm a diagnosis of a suspected genetic disorder