2A - Cell Structure And Division Flashcards

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1
Q

What are eukaryotic and prokaryotic cells?

A

• Eukaryotic
- are complex
- animal, plant, fungi organisms consist of them.
- have membrane bound organelles

• prokaryotic
- are smaller and simpler
- bacteria, eg, are prokaryotic cells (are single-celled)
- No membrane bound organelles

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2
Q

What’s a cell-surface/plasma membrane

A

Membrane found on surface of animal cells; inside cell walls of other cell types.
Made up of lipids and protein.

  • regulates movement of substances in/out cell
  • has receptor molecules on it, to respond to chemicals like hormones
  • has antigens that system sees as safe
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3
Q

What’s the description and function the nucleus and its parts?

A

It’s a large organelle surrounded by nuclear envelope (double membrane).
They have many nuclear pores.
It’s filled by the nucleoplasm
Contains chromatin wch condenses into chromosomes (made from protein bound linear DNA)
Has a nucleolus

  • nucleus controls cell activities by controlling DNA transcription.
  • DNA contains instructions to make proteins.
  • pores allow substances (mRNA eg) to move in/out nucleus
  • Nucleolus makes ribosomes (synthesises ribosomal RNA + assembles)
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4
Q

Mitochondrion description and function , including parts

A

They’re usually oval shaped with a double membrane.
The inner membrane is creased to create folds called christae
Christae increase SA so respiration = faster

Inside inner membrane is matrix (contains enzymes for respiration)

  • mitochondria are site of respiration where ATP is produced
  • ATP are energy carrier molecules
  • The more active the cell, the more mitochondria (more energy needed)
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5
Q

What’s Golgi apparatus? And vesicles?

A

A group of membrane-bound flattened sacs (cisternae)

Vesicles (little dots) are often at edges of sacs, produced by Golgi apparatus
Vesicles are small fluid-filled sacs in cytoplasm surrounded by a membrane

  • they modify and package new lipids and proteins/enzymes
  • the vesicles transport and store them.
  • also makes lysosomes.
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6
Q

What’s a lysosome

A

a round organelle surrounded by a membrane - no clear internal structure
It’s a type of golgi vesicle

  • contains lysozymes (digestive enzymes)
  • kept separate from cytoplasm by its membrane
  • uses lysozymes to digest invading cells, or to break down worn cell components.
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7
Q

What are the extra organelles in plant cells

A

They have same organelles as animal cells but with
- a cellulose cell wall (with plasmodesmata; channels to exchange substances with adjacent cells)
- a vacuole
- chloroplasts

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8
Q

What are the structures of algae and fungal cells

A

• Algae
- have all same organelles as plant cells
- they carry out photosynthesis, but can be single-celled or multicellular

• Fungal cells (mushrooms, yeast)
- like plant cells with differences.
- cell walls of chitin not cellulose
- no chloroplasts (don’t photosynthesise)

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9
Q

What’s structure and function of chloroplasts

A

Small flattened structure..
- surrounded by double membrane

  • also has membranes inside (thylakoid membranes)
  • these membranes stack up in some parts of the chloroplast (grana)
  • grana are linked by lamellae (thin flat thylakoid membrane pieces)

The site of photosynthesis
Initial parts of photosynthesis happen in the grana
Then in the stroma (thick fluid in chloroplasts)

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10
Q

Ribosome described and function

A

A very small organelle (floats free or attached to RER)
- made up of proteins and RNA
- no membrane

Site of protein synthesis

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11
Q

Rough endoplasmic recticulum structure and function

A

System of membranes enclosing on fluid-filled space.
Surface covered in ribosomes

Function
- folds and processes (sort and modified) proteins made at ribosomes

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12
Q

Smooth endoplasmic recticulum structure and function

A

Same as rough endoplasmic recticulum (no ribosomes)
—> more tube-like

Synthesises and processes lipids, as function.
Stores and transports lipids too.

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13
Q

What are structure and functions of cell wall and vacuole?

A

•Cell wall
- rigid structure, surrounds plant, algae and fungi cells
- cellulose in plants and algae
- chitin in fungi

As a function, it supports cells, preventing change in shape.

• vacuole
- membrane bound organelle on ONLY plant cell cytoplasm
- cell sap inside (weak sugar and salt solution)
- membrane is called the tonoplast

Function..
Is to maintain pressure in cell to keep it rigid
Stopping plants wilting.
Also involved in isolating unwanted chemicals in cell.

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14
Q

What’s the structure of prokaryotic cells, and its organelle functions

A

They’re smaller and simpler than eukaryotic cells (DIAGRAM NO. 2)

•cell wall
Supports cell preventing shape change
Made of murein, a polymer.
—> It is a glycoprotein (protein with carb attached).

• plasma membrane
Made of lipids and proteins
Controls what exits and enters cell

• cytoplasm
Has no membrane bound organelles
Has smaller ribosomes than those in eukaryotic cells

• flagellum
Long hairlike structure that rotates to make cell move
(Not all have it. Some have more than one)

• no nucleus
- DNA floats free in cytoplasm
It’s circular, a coiled up strand
Not attached to histone proteins

  • plasmids are small dna loops
    Not part of main circular DNA molecule
    Contains genes for antibiotic resistance AND can be passed between prokaryotes
    (Not always present; some have several)

• capsule
Made up of secreted slime
To protect bacteria from attack by cells in immune system
Also helps groups of bacteria stick together

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15
Q

What is cell fractionation

A

It’s the process of separating organelles.
Cell fractionation makes it possible to view organelles under an electron microscope.
There are 3 steps….

  • homogenisation
  • filtration
  • ultracentrifugation
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16
Q

What’s homogenisation

A

It’s breaking up the cells

• it can be done in many ways,
like grinding cells in a blender
Or vibrating the cells.
—> this breaks up plasma membrane and releases organelles into solution

—> Conditions..

  • kept ice-cold
    To reduce enzyme activity that break down organelles.
  • solution shd be isotonic
    Having same conc of chemicals/solute/water pot. As inside cells
    To prevent organelle damage by osmosis
  • maintain pH
    By adding buffer solution
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17
Q

What’s filtration in cells fractionation

A

It’s getting rid of the big chunks

• the homogenised cell solution is filtered
through a gauze to separate
large cell debris or tissue debris (eg, connective tissue from organelles)

The organelles are much smaller than debris so they pass through gauze

18
Q

What’s ultracentrifugation in cell fractionation

A

It’s separating the organelles
After filtering, you have a solution with a mixture of organelles
To separate a particular organelle from others,
You use ultracentrifugation.

• cell fragments pour into a tube, then in centrifuge (machine separates material by spinning)
• it’s spun at a low speed
—> heaviest organelles (nuclei) get flung to bottom of tube by centrifuge
- Forming thick sediment (pellet) at bottom
- Rest of organelles stay suspended in fluid above sediment (supernatant)

• supernatant is drained off, poured into another tube
• and centrifuge spins at higher speed
• again, heaviest organelles (mitochondria now) form a pellet
• supernatant with rest of organelles is drained,
and spun again at higher speed

• process is repeated at higher and higher speeds, until all organelles separated out
• each time, pellet is made up of lighter organelles

19
Q

What’s order of organelles separated in a centrifuge

A

(Order of mass; heaviest to lightest)
- nuclei
- (chloroplasts)
- mitochondria
- lysosomes
- then endoplasmic recticulum and golgi
- ribosomes

20
Q

What is magnification and resolution? 🟡

A

Magnification
- how much bigger the image is than the specimen
Mag = image size / actual size 🟡

Resolution
—> how detailed image is
- how well something has the ability to distinguish
- between two points close together.
If a microscope can’t separate two points, increasing mag won’t help

21
Q

What are the differing features of light and electron microscopes

A

• light microscopes
- use light to form images (larger wavelength)
- have max resolution of 0.2μm
- max. Magnification is abt x1500
- specimens dead or alive
- cheap
-
- so can’t see organelles smaller than this (ribosomes, ERs, lysosomes)
- might make out mitochondria and nucleus

• electron microscope
- use electrons to form image (small wavelength)
- high resolution (0.0002μm) , so more detailed images
- max. magnification of 1500000x
- specimens killed in prep
- expensive

22
Q

What are differences between TEMs and SEMs?

A

• Transmission Electron Microscope (TEM)

  • use electromagnets to focus electron beams, transmitted through specimen
  • denser parts of specimen absorb more electrons.
    This means artefacts (dark blobs) produced
    -
  • only used in thin specimens
  • good resolution (can see internal organelle struc)
    -black and white images
  • in vacuum (not living)


• Scanning Electron Microscope (SEM)

  • Scan electron beams across specimen
    This is knocking off electrons from specimen
    Then gathering them in a cathode ray tube to form images
  • images show specimen surface in 3D (and in false colour)
  • can be used in thick specimens
  • lower resolution images than TEMs
  • Specimens frozen in liquid nitrogen and in vacuum
23
Q

What’s are SEMs and TEMs used for

A

TEM
To see inside cells and their organelles
And specimens’ sections

SEM
Used to see cell surfaces
Or 3D structures

24
Q

How can you use a graticule and micrometer to calculate cell sizes?

A

The eyepiece graticule and stage micrometer are like rulers.
—> eyepiece graticules are fitted onto the eyepiece
(Transparent ruler with numbers + no units)

—> stage micrometers are placed on the stage
(Microscope slide with an accurate scale - has units)
— Used to work out value of divisions on eyepiece graticule
- at a particular magnification

It means if you take stage micrometer away and replace
With slide containing tissue sample
You’ll be able to measure size of cells.

25
Q

What is an example of using a graticule and micrometer to find cell size?

A
  1. Line up eyepiece graticule and stage micrometer
    —> each division on stage micrometer is 0.1 mm long

..

  1. (no. 3) At this magnification, 1 division on stage micrometer
    Is same as 4.5 divisions on eyepiece graticule
  2. To work out size for 1 division on eyepiece graticule
    Divide 0.1by 4.5
    1 division on EG = 0.022mm
  3. If you look at a cell under a microscope at this magnification
    And it’s 4 divisions long, it measures
    4 x 0.022= 0.088
26
Q

What are specialised cells and describe epithelial cells

A

In multicellular eukaryotic organisms, cells are specialised to do specific functions
A cell’s struc (shape, organelles) helps carry out its function
So depending on its job, they can look different to regular animal cells

Eg. Epithelial cells…
- walls of small intestine have finger like projections called villi
- this increases SA for absorption
- epithelial cells on surface of villi have folds in cell membrane called micro villi
- microvilli increase SA even more

  • also have lot of mitochondria, to provide energy
    to transport digested food molecules into cell
27
Q

What are artefacts

A

They are things you can see down the microscope
That aren’t part of the specimen you’re looking at
—> can be anything from dust, air bubbles and fingerprints
—> to inaccuracies caused by squashing/staining samples

Artefacts are usually made during preparation of slides (shdnt be there)
You must tprpare specimen on slides carefully (to avoid this)

..

Are common in electron micrographs;
specimens needs lots of preparation before you can see them

First scientists could only distinguish between artefacts
and organelles by repeatedly preparing specimens in different ways
—> in an objects is seen with one preparation technique but not another
- it’s more likely to be an artefact than an organelle

28
Q

what is interphase

A

where mitosis starts - the cell doesnt divide here.
- interphase is a period of lots of cellular activity
- involving DNA replication
- the two copies of DNA (after replication) remain joined at the centromere
-
- also organelles are replicated

29
Q

what does mitosis produce

A
  • two daughter cells (same number of chromosomes as each other and parent cell)
  • genetically identical
  • diploid cells
30
Q

prophase?

A

chromosomes start to become visible (from short thin threads = later shorten and thicken / condense)
- nucleolus disappears; nuclear envelope breaks down
- chromosomes drawn to equator of cell by spindle fibres

animal cells have two cylindrical organelles called centrioles
- move to opposite ends / poles of the cell
- from each of the centrioles, spindle fibres develop, attached (span cell from pole to pole)
-> spindle fibres=spindle apparatus collectively

31
Q

prophase in plants

A

plant cells lack centrioles but do develop spindle appartaus
(so centrioles arent essential for spindle fibre formation)

32
Q

metaphase

A

the chromosomes are noe seen to be made of two chromatids (each are indentical copies of dna from parent cell)
- both joined by centromere

some microtubules from the poles are attached
so pulled along the spindle apparatus to line up across cell equator

33
Q

anaphase?

A

centromeres divide and spindle fibres pull individual chromatids apart.
as are single, now referred to as chromosomes

energy for process from mitochondria (gather round spindle fibres)

if cells treated with chemicals that destroy spindle, the remain at equator, not dividing

34
Q

telophase?

A

chromosomes, wch are at the poles, become longer and thinner, leaving wide spread chromatin
- spindle fibre disintegrates
- nuclear envelope and nucleolus reform

35
Q

cytokinesis

A

cytoplasm divides, making two daughter cells

36
Q

mitosis importance

A
  • growth (mitosis ensures every body cell has the same dna. Involved in development of tissues)
  • repair (if cells are damaged or die, the new cells have an identical structure and function)
  • reproduction (single celled organisms divide by mitosis to give two new organisms)
37
Q

What is cancer

A

Group of diseases caused by a growth disorder in cells
(Result of damage to the genes that regulate mitosis and cell cycle)

This leads to uncontrolled division of cells.
A group of these cells produced from uncontrolled division
Form a tumour - wch can be benign or malignant (cancerous)

Cancerous tumours invade surrounding tissue

38
Q

What are some cancer treatments

A

Some treatments are designed to control cell division rate in tumours
By disrupting the cell cycle, killing tumours cells
Also can kill normal body cells dividing too rapidly, but more likely to kill tumour cells
- some cell cycle targets of cancer treatments..

  1. G1 (cell growth/protein production)
    - some chemical drugs (chemotherapy) prevent
    - synthesis of enzymes needed in DNA replication
    - if not produced the cell is unable to enter synthesis (S) phase
    - disrupting cell cycle, forcing it to kill itself
  2. S phase (dna replication)
    - radiation and some drugs damage DNA
    - at several points in cell cycle (including just before and during S phase)
    - dna in cell is checked for damage
    - if severe damage detected, the cell will kill itself

Both stopping further tumour growth

39
Q

Which processes ensure cell division is controlled

A

Before a cell divides , dna is checked to ensure it has been replicated correctly
Chemical signals then tell a cell to stop or start division
Neighbouring cells communicate with dividing cells to regulate growth too

If any of these don’t happen, a cell will divide uncontrollably
Leading to cancer

40
Q

(PRACTICAL 🚨) how are root tips stained and squashed to observe mitosis

A

Precautions
- wear gloves and lab coat when handling stains
- use safety goggles when handling acid

  1. Cut 1cm from tip of a growing root like onion
    It’s too as that’s where growth occurs/mitosis takes place
  2. Prepare boiling tube 1M HCl; put in water bath 60°C
  3. Transfer root tip into boiling tube (incubate 5 mins)
  4. Use pipette to rinse rinse root tip with cold water; dry with paper towel
  5. Place root tip on slide; cut 2mm from its tip
  6. Use mounted needle to break root tip open, spreading out cells so specimen’s thin
  7. Add a few drops of toluidine blue stain (makes chromosomes easy to see under microscope)
  8. Place cover slip over cells squash tissue by pushing firmly
    Making tissue thinner so light can pass; don’t smear slip - chromosomes will damage
  9. Now you can see stages of mitosis under optical microscope

(DIAGRAM NO,4)

41
Q

(PRACTICAL 🚨) Following up from root tip prac, how to observe cells with optical microscope

A
  1. Clip slide you’ve prepared under the stage; turn light on
  2. Select lowest magnification objective lens
  3. Use the coarse adjustment knob to bring up stage (just below lens)
  4. Look down eyepiece (ocular lens); turn coarse adjustment knob away from objective lens until image is roughly in focus
  5. Adjust focus with fine adjustment knob until image is clear
  6. Upon increasing magnification, swap to higher power objective lens and refocus

If asked to draw cells undergoing mitosis
- write magnification of specimen
- also label drawing

42
Q

What actually is mitotic index

A

= number of cells with visible chromosomes/undergoing mitosis
/total number cells observed

Let’s you find how quick tissue is growing
- a plant root tip is constantly growing, so you’d find a high mitotic index
- in other samples, a high mitotic index indicates tissue is repairing or cancerous growth