24. Properties of viruses Flashcards

1
Q

enveloped viruses are disrupted by ___

A

acid
detergents
drying
heat

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2
Q

what are the morphological groups of viruses?

A
  • naked icosahedral
  • enveloped icosahedral
  • naked helical
  • enveloped helical
  • complex
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3
Q

viruses are only visible under a __

A

electron microscope

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4
Q

how do you cultivate viruses?

A

living host cells

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5
Q

and enveloped virus is released by:

A

budding and cell lysis

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6
Q

cell lines

A

diploid, 50-70 passages

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7
Q

what happens to viral genomes of DNA viruses?

A

they remain in the infected cell, most reside in the nucleus (except pox viruses)

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8
Q

a naked capsid is stable to

A
  • temperature
  • acid
  • proteases
  • detergents
  • drying
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9
Q

eclipse period

A

time from first infection to intracellular virus

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10
Q

almost all viruses contain either __ or __ with the exception of __

A

DNA, RNA, CMV

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11
Q

steps in viral replication

A
  • recognition
  • attachment and penetration
  • uncoating
  • macromolecular synthesis
  • early mRNA codes for non-structural proteins
  • late mRNA codes for structural proteins
  • posttranslational modification of proteins
  • assembly of viral components
  • budding of enveloped viruses
  • release of virus
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12
Q

can cells replicate RNA?

A

no, RNA viruses must encode an RNA-dependent RNA polymerase

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13
Q

most RNA viruses replicate in __

A

the cytoplasm

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14
Q

enveloped virus components:

A

membrane
lipids
proteins
glycoproteins

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15
Q

viruses that don’t use the cell polymerase __

A

carry their own polymerase

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16
Q

positive vs. negative strand RNA

A

positive- can be directly transcribed into a protein

negative- has to be transcribed by an enzyme into a positive strand

17
Q

what enzymes do viruses have for energy metabolism?

A

none

18
Q

established cell lines

A

heteroploid, continuous passage in vitro

19
Q

what do DNA polymerases require in order to replicate the viral genome?

A

a primer

20
Q

early genes tend to encode:

A

DNA-binding proteins

21
Q

late genes encode:

A

structural proteins

22
Q

structure of a virus

A

genome in a protective protein shell, capsid shell may be enclosed within an envelope that contains both protein and lipid

23
Q

naked helical viruses only exist in __

A

plants

24
Q

primary cell culture

A

diploid, 1-2 passages, taken from living things

25
Q

latent period

A

the period from intracellular infection to extracellular or released virions

26
Q

hemadsorption

A

test for certain viruses: add erythrocytes, measles virus releases hemaglutinin causing agglutination

27
Q

a naked capsid is released by:

A

lysis of the cell