2.4 Acid Fast Staining

Question 1

Acid fast staining is an important tool in the identification of these two bacteria.

Answer 1

Mycobacterium tuberculosis
Mycobacterium leprae

Question 2

A complex lipid with fatty acids and alcohols that have hydrocarbon chains up to 80.

Answer 2

Mycolic acid

Question 3

Mycolic acid is a complex lipid with fatty acids and alcohols that have hydrocarbon chains up to ___.

Answer 3

80

Question 4

Which step of the staining process do acid fast negative bacteria become colorless?

Answer 4

Decolorization

Question 5

Primary stain?

Answer 5

Carbolfuschin red

Question 6

Decolorizer is made of?

Answer 6

HCl and alcohol

Question 7

What is the counter stain?

Answer 7

Methylene blue

Question 8

What is the color of acid fast negative bacteria after methylene blue?

Answer 8

Blue

Question 9

When heating the stain, how long do we let it heat?

Answer 9

5 minutes

Question 10

What are the percentages of HCl and ethanol in the decolorizer?

Answer 10

3%
95%

Question 11

Can we blot dry the Ziehl-Neelsen method?

Answer 11

No

Question 12

The heat method of AFS

Answer 12

Ziehl-Neelsen method