2.1 Bacterial Smear

Question 1

A dried preparation of bacterial cells o’ a glass slide

Answer 1

Bacterial Smear

Question 2

The bacteria should be evenly spread out on the slide. T or F?

Answer 2

T

Question 3

Step 1 for liquid?

Answer 3

Shake the culture tube to suspend organisms.

Question 4

Step 2 for liquid?

Answer 4

Heat loop to red-hot

Question 5

Step 3 for liquid?

Answer 5

Remove the culture tube cap and flame its neck

Question 6

Step 4 for liquid?

Answer 6

Remove a loopful of organisms from the tube. Do not touch the sides.

Question 7

Step 5 for liquid?

Answer 7

Flame the mouth of the tube.

Question 8

Step 6 for liquid?

Answer 8

Return the culture tube’s cap.

Question 9

What does the target shape look like?

Answer 9

Oval, thumb-shaped

Question 10

Which comes first, air drying or heat fixing?

Answer 10

Air drying

Question 11

How are you supposed to handle clean slides?

Answer 11

Handle it by the edges

Question 12

Why do we have to cool the loop before inserting to the medium?

Answer 12

To prevent splattering of the medium