22. Regulation of skeletal muscle mass Flashcards

1
Q

What is the approximate rate of protein intake/oxidation and synthesis/breakdown?

A
Intake/oxidation = 80g/day
Synthesis/breakdown = 300g/day
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2
Q

What does more 13CO2 in breath mean?

A

More oxidation of infused tracer.

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3
Q

How do you measure whole body protein synthesis & breakdown?

A

Using stable isotope methodology, we can measure muscle protein synthesis rates by determining the incorporation of labelled AA into protein.

*if you know oxidation, you can calculate synthesis

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4
Q

%/day of protein synthesis in different tissues

A

1.15% muscle protein will be synthesised & broken down daily.

In liver, 12% will be. Therefore in 7-8 days, you will have a completely new liver.

Taking into consideration the mass, the whole body turnover % is 29% and 11% for liver.

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5
Q

Centrifugation steps select for……during isolation of different protein fractions from muscle.

A

Centrifugation steps select:

  • mitochondria
  • sarcoplasmic proteins (eg. signaling proteins)
  • myofibrillar proteins
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6
Q

Describe muscle protein synthesis in different protein fractions in muscle. (mt vs. contractile)

A

Mitochondrial protein turnover is higher than contractile (myosin heavy chain) protein turnover.

Mito FSR > MHC FSR

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7
Q

Is protein synthesis & breakdown constant?

A

Nope, they are not constant during the day. Highly responsive to:

  • food intake (protein, CHO, fat)
  • exercise
  • inflammatory signals
  • hormones, etc.

Protein synthesis is increased during post-exercise recovery and decreased during exercise.

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8
Q

Effects of exercise on mixed muscle protein synthesis in trained & untrained individuals.

A

Mixed muscle protein synthesis is reduced during exercise, but increased for >24 hours post-exercise in untrained individuals.

Trained have a more acute response, but return to baseline much quicker than untrained ind.

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9
Q

What increases the myofibrillar and sarcoplasmic protein synthesis rates (FSR) following exercise?

A

Resistance exercise increases myofibrillar and sarcoplasmic protein synthesis.

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10
Q

Following exercise training, what is the acute response to exercise regarding mt & myofibrillar protein synthesis (FSR)?

A

Mito FSR > myo FSR

Exercise training alters the acute response to exercise.

myofibrillar: (resistance > endurance)
mitochondrial: (endurance > resistance)

This must mean that different signaling pathways are activated.

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11
Q

How is protein synthesis mainly regulated & how can you increase protein synthesis?

A

Through rate of transcription and initiation of binding of ribosome to start codon in mRNA
Also due to energy demanding amino acids and ATP/GTP.

Increased transcription levels or translation levels.

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12
Q

What drives the increase in MPS after exercise?

A

Increased translation seems to drive increased MPS after exercise.

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13
Q

Translation initiation

A

Initial binding of pre-initiation complex to mRNA

Binding of first tRNA to ribosomal subunit (methionine start amino acid)

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14
Q

What is the most responsive pathway to exercise & nutrition?

A

mTOR protein synthesis pathway

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15
Q

What is the effect of resistance exercise on signalling?

A

During exercise, AMPK activity is increased while mTOR signaling is reduced.

However, post exercise, mTOR signaling increases to promote protein synthesis.

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16
Q

What is S6K1 and effect of exercise on S6K1 is…

A

S6K1 is a downstream factor of mTOR pathway.

Effect of exercise on S6K1 is fibre-type specific.

17
Q

Rapamycin and mTOR signaling

A

Rapamycin administration in humans blocks the contraction-induced increase in skeletal muscle protein synthesis.

Drug binds to mTOR & inhibits its activity

18
Q

Describe the muscle wasting process with respect to aging.

A

With increasing age:

  • increase in fat mass
  • decrease in muscle mass.

Sarcopenia is a SLOW process, muscle loss is 8-10% per decade.

Wasting due to reduced anabolic response to food intake/exercise

19
Q

Describe the anabolic resistance in the elderly.

A

Protein synthetic response to exercise is reduced in the elderly.

Protein synthesis rates after physical activity and protein intake are significantly reduced in elderly humans.

20
Q

Anabolic resistance following bed-rest.

A

7 days of bed rest reduces anabolic response to AA intake.

*inactivity changes the activity of mTOR pathway

21
Q

Describe the anabolic resistance in cancer.

A

The increase in protein synthesis after food intake is blunted in cancer patients.

Factors from tumour increase leg proteolysis more so than in post-op and control/non cancer state.

22
Q

What are the pathways that regulate protein synthesis?

A
  1. mTOR pathway

2. translation initiation