2.1.4 Enzymes Flashcards

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1
Q

What is an enzyme?

A
  • A globular protein with complex tertiary structure (some have a quaternary structure)
  • Biological catalyst
  • Speeds up a reaction without being used up
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2
Q

How do enzymes affect the activation energy?

A

They lower it

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3
Q

What happens when an enzyme is catalysing a breakdown?

A
  • Fitting into the active site puts a strain on the bonds of the substrate
  • They can be broken more easily
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4
Q

How are two substrate molecules joined?

A
  • Enzyme holds them together
  • They can bond more easily
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5
Q

What is intracellular enzyme action?

A

When enzymes are made and retained inside the cell
e.g. catalase, polymerase, lysosomal enzymes, ATPase, ATP synthase

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6
Q

What is catalase?

A
  • An intracellular enzyme
  • Breaks down hydrogen peroxide into water and oxygen
  • 2H2O2 –> O2 + 2H2O
  • Found in peroxisomes (vesicles in liver cells)
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7
Q

What is extracellular enzyme action?

A
  • Secreted by cells and work outside of the cells
    E.g. amylase
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8
Q

What is amylase?

A
  • An extracellular enzyme
  • Works outside of cells in saliva to catalyse the hydrolysis of starch into maltose
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9
Q

What is trypsin?

A
  • An extracellular enzyme
  • Hydrolyses peptide bonds of large polypeptides into smaller ones
  • Smaller ones are eventually broken down to amino acids by other enzymes
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10
Q

What enzymes are in fungi?

A
  • Extracellular
  • Cells in fungal hyphae synthesise enzymes that are secreted outside the cell to digest organic matter
  • Absorbed and used for growth
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11
Q

What is the structure of an enzyme?

A

Globular protein:
- 3D shape
- Almost spherical
- Soluble in water

Active site:
- Specific shape
- Where substrate binds to

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12
Q

What is the lock and key model?

A
  • The old model
  • Enzymes will only catalyse certain reactions because the active site is a specific shape to fit the substrate
  • There is no change as active site is exactly complimentary
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13
Q

What is the induced fit model?

A
  • New model
  • Enzyme isn’t complimentary to the substrate
  • Enzyme changes shape slightly as the substrate binds
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14
Q

How does temperature affect enzyme activity?

A
  • At low temperature, enzyme is deactivated (temporary)
  • As temperature increases so does kinetic energy
  • The number of successful collisions with the active site increases the rate of reaction
  • Optimum temperature is where the enzyme activity is at its highest as there will be the maximum amount of enzyme-substrate complexes
  • When temperature is too high, vibrations break bonds that hold enzyme together so enzyme denatures and activity decreases
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15
Q

What is denaturation?

A
  • Irreversible change to the tertiary structure of the active site
  • No longer complementary to substrate
  • No E-S complezes
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16
Q

How are enzymes in thermophiles adapted?

A
  • Thermophiles live in very hot environments
  • Enzymes have more hydrogen bonds and disulfide bridges in the enzyme protein molecules
  • More resistant to temperature rises
  • Cyanobacteria live between 40 and 85 degrees
  • Spirochetes live between 40 and 73 degrees
17
Q

What is Q10?

A

A factor by which the rate of enzyme activity increases when the temperature is raised by 10 degrees

18
Q

What is the formula for Q10?

A

Q10 = rate at higher temperature/rate at lower temperature

19
Q

How does pH affect enzyme activity?

A
  • As you move away from the optimum temperature, enzymes start to denature and activity decreases
  • H+ and OH- ions break the ionic and hydrogen bonds that hold the enzymes tertiary structure in place
  • No longer able to form E-S complexes
  • Either side of the optimum is permanent denaturation
  • Optimum pH is the pH the enzyme works best at
20
Q

What are the optimum pHs of enzymes?

A
  • Pepsin is in the stomach with HCl so optimum is low (1-2)
  • Amylase is in the mouth so needs to be neutral (6-7)
21
Q

How do you control the pH in an investigation?

A

Buffer solutions

22
Q

How does enzyme concentration affect enzyme activity?

A
  • As enzyme concentration increases, rate of reaction increases as there are more active sites
  • Substrate concentration is a limiting factor so eventually increasing enzyme concentration doesn’t increase ROR
23
Q

How does substrate concentration affect enzyme activity?

A
  • As substrate concentration increases, the rate of reaction increases
  • More active sites are engaged
  • More successful collisions with active sites
  • Point of saturation (Vmax) is when all the active sites are filled
  • Past this, enzymes concentration is a limiting factor so ROR doesn’t increase (graph plateaus)
24
Q

What is a cofactor?

A

A non-protein which binds to enzymes to make them work

25
Q

What is an inorganic cofactor?

A
  • An inorganic ion or molecule
  • Helps the enzyme and substrate to bind together but doesn’t directly participate in the reaction
  • Not used up or changed
  • Not inhibitory and enables the reaction

E.g. Chloride ions (Cl-) are cofactors for amylase

26
Q

What are organic cofactors/coenzymes?

A
  • Participate in the reaction and are changed
  • They often act as carriers, moving chemical groups between enzymes
  • Continually recycled during the process

E.g. NAD

27
Q

What is a prosthetic group?

A
  • A cofactor that is tightly bound to an enzyme

E.g. Zinc ions (Zn2+) are a prosthetic group for carbonic anhydrase so are a permanent part of the enzyme’s active site

28
Q

What are competitive inhibitors?

A
  • Similar shape to active site and substrates
  • Bind to the active site but no reaction takes place
  • This blocks the active site and leads to a slower reaction

E.g. Malonate is a competitive inhibitor for succinate dehydrogenase

29
Q

What are non-competitive inhibitors?

A
  • These bind to the allosteric site
  • Causes a change in shape of the active site
  • The substrate molecule can no longer fit so enzyme-substrate complexes are no longer able to be formed

E.g. Copper sulfate is a non-competitive inhibitor for catalase

30
Q

How do the graphs of inhibitors compare?

A
  • A competitive inhibitor will have a slower rate of reaction but still reach the same end point
  • A non-competitive inhibitor has a slower rate of reaction and lower end point as the enzyme’s active site is permanently damaged
  • All 3 lines start from the same point
31
Q

What is a reversible inhibition?

A
  • Inhibitor can be removed
  • Due to weaker hydrogen bonds or weak ionic bonds
32
Q

What is a non-reversible inhibition?

A
  • Inhibitor cannot be removed easily
  • Due to strong covalent bonds
33
Q

What do metabolic poisons do?

A

They interfere with metabolic reactions in cells

34
Q

What is cyanide?

A
  • Non-competitive inhibitor of cytochrome C oxidase
  • Involved with respiration
35
Q

What is arsenic?

A
  • Non-competitive inhibitor of pyruvate dehydrogenase
  • Involved with glycolysis
36
Q

What is malonate?

A
  • Competitive inhibitor of succinate dehydrogenase
  • Involved with the kreb cycle