2.1.4 Enzymes Flashcards
What is an enzyme?
- A globular protein with complex tertiary structure (some have a quaternary structure)
- Biological catalyst
- Speeds up a reaction without being used up
How do enzymes affect the activation energy?
They lower it
What happens when an enzyme is catalysing a breakdown?
- Fitting into the active site puts a strain on the bonds of the substrate
- They can be broken more easily
How are two substrate molecules joined?
- Enzyme holds them together
- They can bond more easily
What is intracellular enzyme action?
When enzymes are made and retained inside the cell
e.g. catalase, polymerase, lysosomal enzymes, ATPase, ATP synthase
What is catalase?
- An intracellular enzyme
- Breaks down hydrogen peroxide into water and oxygen
- 2H2O2 –> O2 + 2H2O
- Found in peroxisomes (vesicles in liver cells)
What is extracellular enzyme action?
- Secreted by cells and work outside of the cells
E.g. amylase
What is amylase?
- An extracellular enzyme
- Works outside of cells in saliva to catalyse the hydrolysis of starch into maltose
What is trypsin?
- An extracellular enzyme
- Hydrolyses peptide bonds of large polypeptides into smaller ones
- Smaller ones are eventually broken down to amino acids by other enzymes
What enzymes are in fungi?
- Extracellular
- Cells in fungal hyphae synthesise enzymes that are secreted outside the cell to digest organic matter
- Absorbed and used for growth
What is the structure of an enzyme?
Globular protein:
- 3D shape
- Almost spherical
- Soluble in water
Active site:
- Specific shape
- Where substrate binds to
What is the lock and key model?
- The old model
- Enzymes will only catalyse certain reactions because the active site is a specific shape to fit the substrate
- There is no change as active site is exactly complimentary
What is the induced fit model?
- New model
- Enzyme isn’t complimentary to the substrate
- Enzyme changes shape slightly as the substrate binds
How does temperature affect enzyme activity?
- At low temperature, enzyme is deactivated (temporary)
- As temperature increases so does kinetic energy
- The number of successful collisions with the active site increases the rate of reaction
- Optimum temperature is where the enzyme activity is at its highest as there will be the maximum amount of enzyme-substrate complexes
- When temperature is too high, vibrations break bonds that hold enzyme together so enzyme denatures and activity decreases
What is denaturation?
- Irreversible change to the tertiary structure of the active site
- No longer complementary to substrate
- No E-S complezes