2.1.1 Cell structure Flashcards
Magnification of light microscope?
1500x
Resolution of light microscope?
200nm
Magnification of transmission electron microscope (TEM)?
500,000x
Resolution of transmission electron microscope (TEM)?
0.5nm
What does a TEM show in a cell?
2D black and white images of the inside of a cell and smaller organelles
Magnification of a scanning electron microscope (SEM)?
100,000x
Resolution of a scanning electron microscope (SEM)?
3-10nm
What does a SEM show in a cell?
3D black and white images of the surface of the specimen
What does a laser scanning confocal microscope show in a cell?
3D coloured images of cells (due to fluorescent tag) which can be living and dead
mm —> μm —> nm
x1000 x1000
What is magnification?
How many times larger the image is than the actual size of the object being viewed
What is resolution?
The ability to see individual objects as separate entities
Magnification = __________ ÷ ____________
Image size ÷ Actual size
(ensure units are the same!)
What are prokaryotes?
Organisms which do NOT have membrane bound organelles
What are eukaryotes?
Organisms which DO have membrane bound organelles
Differences in DNA between prokaryotes and eukaryotes?
Prokaryote
- Circular
- Extra DNA: plasmids
Eukaryotes
- Linear
- Extra DNA: mitochondria and chloroplasts
Difference in ribosomes of prokaryotes and eukaryotes
Prokaryotes - smaller 70S
Eukaryotes - larger 80S