2.1 Cell structure Flashcards

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1
Q

Magnification and resolution of a light microscope

A

x1500
200 nm

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2
Q

Magnification and resolution of a TEM

A

> x500,000
0.5 nm

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3
Q

Magnification and resolution of a SEM

A

x500,000
3-10 nm

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4
Q

What is the image from a TEM

A

2D
Black and White
Organelles visible

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5
Q

What is the image from an SEM

A

3D
Black and White
Cell surface visible

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6
Q

Advantages/Disadvantages of light microscope

A

Cheap
Easy to use
Can view live cultures / tissues

2D images only
cannot view most organelles

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7
Q

Advantages/Disadvantages of TEM

A

More organelles can be seen
Very high magnification + resolution

Dead specimen
Very thin specimen
Lengthy treatment to produce thin specimens

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8
Q

Advantages/Disadvantages of SEM

A

External 3D structure

Lower resolution than TEM
Dead specimens

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9
Q

Why is staining used?

A

Increases the contrast between organelles so differnet organelles can be viewed

Increases the contrast so biological molecules can be seen

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10
Q

Function of the nucleus

A

Contains DNA
Controls production of mRNA
Starts mitosis

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11
Q

Function of nucleolus

A

Manufactures ribosomal RNA and assembles the ribosomes
Ribosomes pass through nuclear pores

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12
Q

Function of the Nuclear envelope

A

Double membrane
Contains nuclear pores - mRNA and ribosomes pass out and nucleotides pass in

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13
Q

Function of Rough Endoplasmic Reticulum

A

Transports proteins made on attached ribosomes

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14
Q

Function of Smooth Endoplasmic Reticulum

A

Lipid, carbohydrate and steroid synthesis - stored in the SER and transported when needed

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15
Q

Function of the Golgi apparatus

A

Modifies proteins and lipids before packaging them into Golgi vesicles

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16
Q

Function of ribosomes

A

Site of protein synthesis
Made of two subunits
80s in larger ribosomes in eukaryotes
70s in prokaryotes

17
Q

Function of Mitochondria

A

Site of respiration
Inner membrane contains enzymes which catalyse aerobic reactions which produce ATP

18
Q

Function of lysosomes

A

Specialised vesicles formed by the Golgi surrounded by a single membrane
Contains digestive enzymes:
–> Breaks down material ingested by phagocytes
–> Release hydrolytic enzymes outside of cell
–> Digests worn out cells - autophagy
–> Role in apoptosis

19
Q

Function of Chloroplasts

A

Site of Photosynthesis

20
Q

Function of the Plasma membrane

A

Regulates the movement of substances in and out of the cell
Receptor molecules allows it to respond to chemicals e.g. hormones

21
Q

Function of centrioles

A

Self-replicating
Nine bundles of microtubules
Forms spindle fibres for mitosis /meiosis

22
Q

Function of Cell wall

A

Cellular support and structure
Made of polysaccharide cellulose

23
Q

Function of Flagella

A

Microtubule pairs slide over each other causing flagella to beat
Moves the cell
–> e.g. the sperm tail

24
Q

Function of Cilia

A

Projections of cell membrane made of microtubules
Move an entire organism or cell i.e. mucus

25
Q

Function of Microvilli

A

Increases SA of cell surface membrane - increased rate of exchange

26
Q

Function of permanent vacuole

A

Keeps cell pressurised

27
Q

What is the importance of the cytoskeleton?

A

Network of protein fibres
Mechanical strength and shape
Holds organelles in place
Move organelles around the cell using ATP