20 ETC AND OXP

Question 1

Provide an overview of electron transport and oxidative phosphorylation in the mitochondria

Question 2

What are Redox reactions?

Question 3

Explain oxidation and reduction

Question 4

What is standard reduction potential?

Question 5

calculation of electrochemical cell potentials

Question 6

calculating ‘delta G’ from cell potentials

Question 7

Explain the process of oxidizing NADH by Complex I NADH-CoQ oxidoreductase (FMN, iron-sulfur clusters, ubiquinone aka CoQ, proton pumping)

Question 8

Explain the process of oxidizing succinate into fumarate by Complex II succinate-CoQ oxidoreductase (iron-sulfur clusters, cytochromes, link to citric acid cycle)

Question 9

Explain the process of oxidizing CoQH(sub2) by Complex III CoQH2-cytochrome c oxidoreductase (cytochromes, cytochrome c, proton pumping) Do not worry about the small details

Question 10

Explain the process of oxidizing reduced cytochrome c by Complex IV cytochrome c oxidase (cytochrome a, cytochrome a3, Cu2+ ions, molecular oxygen (Finally!), proton pumping)

Question 11

Explain how chemiosmotic coupling mechanism provides the energetics for the formation of ATP (proton gradient, ATP synthase, conformational changes: Loose tight →open, ADP + P(sub i) à ATP)

Question 12

Explain how uncouplers inhibit phosphorylation of ADP without affecting electron transport

Question 13

Explain how respiratory inhibitors can act as poisons

Question 14

Explain the significance of the P/O ratio in calculating the amount of ATP formed per NADH or FADH2 molecule

Question 15

Calculate the amount of ATP that can be formed from glycolysis, pyruvate to AcetylCoA, and citric acid cycle

Question 16

explain the glycerolphosphate shuttle

Question 17

explain the malate-aspartate shuttle