2 - Structure and Recognition of DNA Flashcards
1
Q
polymeric structure of nucleic acids
A
- DNA is stable
- RNA is unstable because its prone to hydrolysis
2
Q
nucleoside vs nucleotide
A
nucleosides do not have phosphate group
3
Q
locked nucleic acid (LNA)
A
- synthetically modified DNA analog
- extra bridge ring to lock ribose sugar to form DNA complex
- stabilized and will not be digested
4
Q
mipomersen
A
- FDA approved oligonucleotide drug
- decrease in levels of bad cholesterol (apo B, LDL, and total cholesterol)
- synthetically modified in order to be more effective at decreasing cholesterol levels
5
Q
DNA structures proposed
A
- correct: bases in the centre stacked
- incorrect: phosphates in the centre stacked
6
Q
DNA melting
A
- strands are held together by non-covalent bonding (hydrogen bonding)
- complementary bonding allows strands to reconnect when cooled
7
Q
DNA sequences or individuals recognized through:
A
- major groove interactions
- minor groove interactions
- local unwinding to reveal bases
- individual base flipping out of helix
8
Q
major groove-protein interactions
A
cAMP interacts with the major grooves that lines up the acceptors and donors to the protein
9
Q
minor groove binders
A
Peter Dervan utilized the pattern of H-bond donors and acceptors to design molecules that bind to DNA grooves
10
Q
base flipping
A
uracil-DNA glycosylase (UDG) monitors uracil DNA and will flip it out
