2. structure and functions in living organisms Flashcards
2.2 -> 2.4, 2.7 -> 2.12, 2.13, 2.15 -> 2.32, 2.33B, 2.34 -> 2.39, 2.49, 2.50, 2.55B, 2.56B, 2.57B, 2.58B, 2.71, 2.72B, 2.74B, 2.75B, 2.77B, 2.79B
fill in the blanks
cells are separated from their surrounding environment by ____ ________, within this, there is _________, within this in eukaryotic cells the following organelles are present: _______, __________ (singular mitochondrion), _________
plant cells contain the following additional structures: ____ ____, _________, permanent _______
cell membrane
cytoplasm
nucleus
mitochondria
ribosomes
cell wall
chloroplasts
vacuole
describe the function of the nucleus
controls the cell, contains DNA
describe the function of the mitochondria
where aerobic respiration takes place, energy is released
describe the function of the ribosomes
site of protein synthesis
describe the function of cytoplasm
where cells chemical reactions takes place
describe the function of the cell membrane
controls what goes in and out of the cell
describe the function of the cell wall
made of cellulose (plants), supports the cell
describe the function of chloroplasts
contains a green pigment (chlorophyll), absorbs light for photosynthesis (making glucose)
describe the function of the vacuole
contains cell sap, help keeps the cells shape
the main structures in animal cells are:
nucleus, cell membrane, mitochondria, ribosomes, cytoplasm
in addition to the structures found in animal cells, what else do plant cells contain?
cell wall (made of cellulose), permanent vacuole (filled with cell sap, pushes the cytoplasm against the cell wall, keeping the cell turgid), and chloroplasts (site of photosynthesis)
what chemical elements are present in carbohydrates?
carbon, hydrogen, oxygen
(CHO)
what chemical elements are present in protein?
carbon, hydrogen, oxygen, nitrogen
(CHON)
what chemical elements are present in lipids (fats and oils)?
carbon, hydrogen, oxygen
(CHO)
how do carbohydrates get broken down?
-> name the molecule enzyme and product
starch -> amylase -> maltose -> maltase -> glucose
how do lipids get broken down?
-> name the molecule enzyme and product
large lipid droplets -> bile -> small lipid droplets -> lipase -> fatty acids and glycerol
how do proteins get broken down?
-> name the molecule enzyme and product
protein -> pepsin/ protease -> peptides -> trypsin (protease) -> amino acids
PRACTICAL
describe the test for reducing sugars:
-add benedicts solution to food sample
-place in 80C water bath for 5 mins
-colour change = blue -> brick red
PRACTICAL
describe the test for starch:
-add iodine solution to food sample
-colour change = orange -> blue black
PRACTICAL
describe the test for protein:
-add biuret solution to food sample
-colour change = blue -> purple
PRACTICAL
describe the test for lipids:
-emulsion test- add ethanol to food sample, shake
-add water, shake
-colour change = colourless -> cloudy white emulsion
what are some safety hazards for each food sample test?
-iodine is an irritant to the eyes, so wear goggles
-same with copper (II) sulfate in biuret solution
-ethanol is highly flammable, so keep away from Bunsen burner
definition of an enzyme
a biological catalyst that speeds up the rate of metabolic reactions without being changed or used up in the process
fill in the blanks: (mechanics of enzyme reaction)
the shape of the ________ is specific / ________ to the ______ ____
substrate
complementary
active site
describe the effect of temperature on enzymes:
-as temperature increases, so does the rate of metabolic reaction, as there is more kinetic energy and therefore more enzyme-substrate collisions occur
-the optimum temperature is 40C- this is when the most collisions happen
-too high temp= too much kinetic energy, active site changes shape, enzymes denature
-remember too low temp does not denature enzymes- instead, low rate of reaction due to the lack of kinetic energy
practical: investigate how enzyme activity can be affected by changes in temperature
- add starch solution to a test tube and heat to a set temperature using a beaker of water + bunsen burner
- add iodine to spotting tile
- use a syringe to add amylase to the starch solution, mix well
- every minute transfer a drop of the solution to the iodine (should turn blue/black)
- repeat until it stops turning blue/ black
- record the time taken
- repeat using a range of temperatures
what is the optimum pH for enzymes?
7 (but some are produced in acidic conditions like the stomach have opH as 2, and some are made in alkaline conditions like duodenum have a higher opH like 8/9)
what happens if the pH is too high or too low for an enzyme
at extreme pH (furthest from optimum) enzymes are completely denatured, so no activity
at optimum pH the rate of reaction is very high
moving away from the optimum pH results in a gradual loss of activity as the active sit becomes distorted
define diffusion
movement of molecules from a high to low concentration down a concentration gradient
define osmosis
net movement of water from a high water potential to a low water potential across a partially permeable membrane
define active transport
movement of molecules against a concentration gradient from a low to high concentration gradient. uses energy
where does diffusion occur?
in the lungs, alveoli, villi
where does osmosis occur?
large intestines, kidney
where does active transport occur?
root hair cells (have lots of mitochondria, so can respire, so have energy for active transport)
explain how the root hair cell takes up nitrate ions from the soil. what would happen if you added air?
by active transport- where the nitrate ions move from a low to high concentration against the concentration gradient, by using energy from respiration
by adding air, it increases oxygen, so increases respiration, so increases energy which is needed to complete active transport
fill in the blanks:
diffusion helps living organisms to:
-obtain many of their __________
-get rid of many of their _____ products
-carry out ___ exchange for __________
requirements
waste
gas, respiration
what would happen if an animal cell is placed in distilled water?
-cell has lower water potential than the solution
-so water moves into the cell via osmosis (high-low)
-cell will swell, may lyse (burst)
what would happen if an animal cell is placed in a strong sugar/salt solution?
-cell has higher water potential than solution
-so water moves out of the cell via osmosis (high-low)
-cell becomes crenated (shrivelled up)
what would happen if a plant cell is placed in a strong sugar/salt solution?
-cell has a higher water potential than the solution
-net movement of water out (loses water via osmosis (high-low))
-vacuole gets smaller and cell membrane shrivels away from cell wall
-becomes flaccid or plasmolysed
what would happen if a plant cell is placed in distilled water?
-cell has a lower water potential than the solution
-net movement of water in (gains water via osmosis (high-low))
-vacuole gets bigger, pushing the cell membrane against the cell wall
-becomes turgid
give one feature of the cell wall which allows the cell to become turgid
its elastic, strong and fully permeable
how does surface area affect the rate of diffusion?
greater the surface area to volume ratio is, the greater the rate of diffusion is
how does distance affect the rate of diffusion?
the smaller the distance, the faster rate of diffusion is (that’s why capillaries and alveoli have walls that are only 1 cell thick)
how does temperature affect the rate of diffusion?
the higher the temperature, the more kinetic energy there is, so the molecules are able to move/ spread faster- therefore increased diffusion rate
how does concentration gradient affect the rate of diffusion?
greater the difference in concentration, the faster the rate of diffusion is
what type of reaction is photosynthesis?
endothermic (chemical reaction that takes in energy from the surroundings- i.e. energy from sunlight is transferred to the chloroplasts)
describe the process of photosynthesis:
energy from sunlight is absorbed by chlorophyll
green plants use this energy to make the carbohydrate glucose from the raw materials carbon dioxide and water
at the same time, oxygen is made and released as a waste product
what is the word equation for photosynthesis?
carbon dioxide + water -> glucose + oxygen
what is the symbol equation for photosynthesis?
6CO2 + 6H2O -> C6H12O6 + 6O2
what are the limiting factors of photosynthesis?
temperature, light intensity, carbon dioxide concentration
how does temperature affect the rate of photosynthesis?
as temperature increases the number of collisions increases, therefore the rate of photosynthesis increases. however, at high temperatures, enzymes are denatured.
how does light intensity affect the rate of photosynthesis?
the more light a plant receives, the faster the rate of photosynthesis up to a certain point
how does co2 concentration affect the rate of photosynthesis?
the more co2 that is present, the faster the reaction occurs
what is the function of the waxy cuticle?
-reduces water loss by evaporation by waterproofing the leaves (protective layer on top)
-barrier to disease causing microorganisms
-contains few stomata- reduce water loss
what is the function of the upper epidermis?
thin and transparent- allows sunlight to reach the palisade mesophyll for photosynthesis
what is the function of the palisade mesophyll?
-column shaped, thin and arranged close together (uniformed)
-contains many chloroplasts and densely packed so that as much light energy can be absorbed as possible for efficient photosynthesis
what is the function of the vascular bundle?
-contains xylem and phloem to transport substances to and from the leaf
-xylem - transports water and mineral salts from the root to leaves (one way)
-phloem - transports sucrose and amino acids around the plant
what is the function of the spongy mesophyll?
contains internal air spaces that increases the surface area to volume ratio for the diffusion of gases (mainly co2)
what is the function of the lower epidermis?
-acts as a protective layer, contains more stomata compared to upper epidermis to regulate loss of water and site of gaseous exchange into and out of the cell (less exposed to light- so less evaporation of H2O)
what is the function of the stomata?
-regulates water loss (transpiration)
-site of gaseous exchange in the leaf
what is the function of the guard cells?
controls the opening and closing of the stomata
what are some adaptations of the leaf?
- leaf = large SA - increases surface area for the diffusion of co2 and absorption of light for photosynthesis
- thin - allows co2 to diffuse to palisade mesophyll quickly
why do plants need mineral ions? which ones do they need most?
for growth
magneisum and nitrates
how do plants obtain mineral ions?
actively absorbed from the soil by root hair cells
why do plants need magnesium? what is its deficiency?
-to make chlorophyll (function)
-causes yellowing of the leaves (deficiency)
why do plants need nitrates? what is its deficiency?
-to make amino acids (function)
-causes stunted growth and yellowing of the leaves (deficiency)