2. DNA Structure and Replication Flashcards
DNA structure, replication and PCR
What makes up a nucleotide?
Sugar, Phosphate and a Base
What is the backbone of DNA?
Deoxyribose Sugar and phosphate
What are the 4 bases of DNA?
A, T, C and G
What is the base pairing rule?
A to T and C to G
Which bonds form between DNA bases?
Hydrogen Bonds
What is the 3’ end?
Where the nucleotide is added
What is the 5’ end?
Where the phosphate is located
Which enzyme controls DNA replication?
DNA Polymerase
Which enzyme joins the okazaki fragments of the lagging strand?
Ligase
In which direction is DNA replicated?
5’ to 3’ direction
What is PCR?
The amplification of DNA molecules
What are some applications of PCR?
Forensic identification, paternity testing, medical diagnosis
What happens when DNA is heated during PCR?
Strands separate
What is a primer?
Short nucleotide sequence of complimentary bases