2. DNA Structure and Replication Flashcards

DNA structure, replication and PCR

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1
Q

What makes up a nucleotide?

A

Sugar, Phosphate and a Base

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2
Q

What is the backbone of DNA?

A

Deoxyribose Sugar and phosphate

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3
Q

What are the 4 bases of DNA?

A

A, T, C and G

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4
Q

What is the base pairing rule?

A

A to T and C to G

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5
Q

Which bonds form between DNA bases?

A

Hydrogen Bonds

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6
Q

What is the 3’ end?

A

Where the nucleotide is added

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7
Q

What is the 5’ end?

A

Where the phosphate is located

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8
Q

Which enzyme controls DNA replication?

A

DNA Polymerase

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9
Q

Which enzyme joins the okazaki fragments of the lagging strand?

A

Ligase

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10
Q

In which direction is DNA replicated?

A

5’ to 3’ direction

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11
Q

What is PCR?

A

The amplification of DNA molecules

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12
Q

What are some applications of PCR?

A

Forensic identification, paternity testing, medical diagnosis

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13
Q

What happens when DNA is heated during PCR?

A

Strands separate

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14
Q

What is a primer?

A

Short nucleotide sequence of complimentary bases

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