[1S] UNIT 1.1 Introduction to Mycology Flashcards
A discipline of Biology that deals with, describes a enormous group of organisms denominated FUNGI
Mycology
highlights the productive or beneficial effects and applications of this field of science particularly in the area of agriculture, biotechnology and environmental biology
Good Mycology
“Dark side” of Mycology; Fungi are implicated as a cause of multiple phenomena that have deleterious effect on environment and health
Bad Mycology
• Transformation and recycling of dead material
• Recycling of carbon and other minerals
• Provide nutrients to the plants
Good Mycology
• Important Biotechnological tool e.g. yeast
• Source of secondary metabolites for production of antibiotics and immunosuppressive drugs (Ex. Cyclosporin A
Good Mycology
• There are significant phytopathogens
• Cause life threatening disease in patients with risk factors
Bad Mycology
• There are significant phytopathogens
• Cause life threatening disease in patients with risk factors
Bad Mycology
BIOLOGICAL RESEARCH
main eukaryotic models in genetics, molecular biology, cell biology, biochemistry and metabolism.
Saccharomyces cerevisiae
BIOLOGICAL RESEARCH
key organism in understanding the mechanism of regulation of the cell cycle
Schizosaccharomyces pombe
NON MOTILE, EUKARYOTIC ORGANISMS, can be single celled or usually are the very complex multicellular organisms
Fungi
A diverse group made up of the classic pathogens, environmental saprobes and parasitic spore-producing eukaryotic organisms that lack chlorophyll. (i.e. Achlorophyllous)
Fungi
• Reside in nature , found in any habitat – on the land, in soil or on plant material rather than in sea or fresh water
• Are obligate or facultative aerobes
Fungi
Are chemotrophic secretes enzymes that degrade a wide range of organic substrates into soluble nutrients which are then transported into the cell through passive absorption or active transport
Fungi
may live as heterotrophs, saprotrophs, and parasitic organisms
Fungi
are subdivided on the basis of their life cycles, the presence or structure of their fruiting body and the arrangement of and type of spores (reproductive or distributional cells) they produce.
Fungi
T/F: Historically, fungus are compared to plants and have also been compared with bacteria
T
SIMILARITIES BETWEEN PLANTS & FUNGI
T/F: Both are PROKARYOTIC organisms
F; EUKARYOTIC
SIMILARITIES BETWEEN PLANTS & FUNGI
T/F:
• Numerous organelles
• Possess cell walls
T
SIMILARITIES BETWEEN PLANTS & FUNGI
T/F:
• Mostly are anchored on soil or other substrates
• Reproduction can be asexual or sexual or both
• Stationary
T
FUNGI VS PLANTS
Multinucleated
Heterotroph
No reproduction by seed
Fungi
FUNGI VS PLANTS
Glycogen is the main storage product
Have chitin in their cell wall
Fungi
FUNGI VS PLANTS
No stem, roots and leaves
Lacks chlorophyll
Fungi
FUNGI VS PLANTS
No stem, roots and leaves
Lacks chlorophyll
Fungi
TYPICAL SIZE
0.4 μm -2 μm in diameter
0.5 μm -5 μm in length
Prokaryotes
TYPICAL SIZE
10 μm -100 μm in diameter
>10 μm in length
Eukaryotes
REPRODUCTION
Asexual (binary fission)
Prokaryotes
NUCLEUS
No nuclear membrane, nucleoid region of the cytosol
Prokaryotes
GENOME
Circular DNA complexed with RNA at mesosome within nucleoid
Plasmid- each carries genes for its own replication; can confer resistance to antibiotics
Prokaryotes
MEMBRANE-BOUND ORGANELLES & CHLOROPLAST FOR PHOTOSYNTHESIS
Absent in all
Prokaryotes
RIBOSOME
Present in all
Prokaryotes & Eukaryotes
PLASMA MEMBRANE
Lacks carbohydrates
Prokaryotes
STEROLS IN CYTOPLASMIC MEMBRANE
Absent except in Mycoplasmataceae
Prokaryotes
ELECTRON TRANSPORT FOR ENERGY
In the cell membrane
Prokaryotes
REPRODUCTION
Sexual and asexual
Eukaryotes
GENOME
Linear DNA complexed with basic histones and proteins in the nucleus
In mitochondria and chloroplasts
Eukaryotes
NUCLEUS
Classic membrane bound nucleus
Eukaryotes
MEMBRANE-BOUND ORGANELLES
Present (e.g. mitochondria, lysosome, ER. Golgi complex, nucleus)
Eukaryotes
RIBOSOME
Present in all
Eukaryotes
CHLOROPLAST FOR PHOTOSYNTHESIS
Present in algae and plants
Eukaryotes
ELECTRON TRANSPORT FOR ENERGY
In the inner membrane of mitochondria and chloroplasts
Eukaryotes
STEROLS IN CYTOPLASMIC MEMBRANE
Present
Eukaryotes
PLASMA MEMBRANE
Also contains glycolipids and glycoproteins
Eukaryotes
CELL WALL, IF PRESENT
Cellulose, phenolic polymers, lignin (plants), chitin (fungi), other glycans (algae)
Eukaryotes
GLYCOCALYX
Present; some animal cell
Eukaryotes
CILIA = Present
PILI & FIMBRIAE = Absent
Eukaryotes
FLAGELLA IF PRESENT
Complex cilia or flagella; composed of MTs and polymers of tubulin with dynein connecting MTs; movement by coordinated sliding microtubules
Eukaryotes
GLYCOCALYX
Present in most as an organized capsule or unorganized slime layer
Prokaryotes
CELL WALL, IF PRESENT
Peptidoglycan in most bacteria
Prokaryotes
FLAGELLA IF PRESENT
Simple flagella; composed of polymers of flagellin; movement by rotary action at the base; spirochetes have MTs
Prokaryotes
- single vegetative cell
- In culture, shows smooth creamy, bacteria like colony without aerial hypha
Yeast
CILIA = Absent
PILI & FIMBRIAE = Present
Prokaryotes
FUNGAL STRUCTURE
- exhibits plasticity
- has turgor pressure
- confers shape to the hypha
Definite, rigid cell wall
FUNGAL STRUCTURE
- protects the protoplast against environmental hazards, osmotic stress
- Acting as a carrier of specific antigen characteristics of the particular cell and playing an important role in cell recognition in various cell interactions.
Cell Wall
- Reproduce by budding or fission
- Can be identified using Biochemical test and Molecular Diagnostic methods
Yeast
- grows in multicellular filaments called hyphae.
- are made up of tubular branches having multiple, genetically identical nuclei, yet form a single organism, known as a colony.
Molds
FUNGAL STRUCTURE
Acting as the site of various extracellular enzymes engaged in the exchange of nutrients and products of metabolism and hydrolysis of cell wall components.
Cell Wall
FUNGAL STRUCTURE
- acts as a filter controlling to some extent materials that enter the fungal protoplast
- Acting as a reservoir of carbohydrates
Cell Wall
Skeletal Components of CW
- Chitin
- ß Glucan
- Mannan
FUNGAL STRUCTURE
consists of water-soluble polysaccharide e.g. α glucan and glycoprotein
Cell Wall Matrix
FUNGAL STRUCTURE
production of this pigment contributes to fungal virulence ; improves resistance to environmental damage such as extreme temperature, UV light and toxins ; and is important for invasion and dissemination.
Miscellaneous Cell Wall Components : Melanin
FUNGAL STRUCTURE
nucleus, mitochondrion, E.R. Golgi bodies, microbodies
Membrane-bound organelles
FUNGAL STRUCTURE
are plaque-like structures embedded in the nuclear envelope for the entire cell cycle in budding yeast and most of the cell cycle in fission yeast. It plays the role of the centrosome by initiating organization of microtubule, particularly during mitosis.
Spindle Polar Bodies (SPBs)
FUNGAL STRUCTURE
vesicles associated with filamentous material, found numerous at the tip of actively growing hypha
Filasomes
A non-motile thallus constructed of apically elongating walled filaments. A web of filaments or hypha constitutes a _______
MYCELIUM
MOLDS
FUNGAL STRUCTURE - MOLD
filamentous tubular structures that grow by elongation (like thread) at the tip ; or by branching that contain numerous nuclei distributed throughout.
Hypha
FUNGAL STRUCTURE - MOLD
refer to the entire , vegetative body of the hypha
Thallus
FUNGAL STRUCTURE - MOLD
partitions or crosswall in hyphal structure
Septum
FUNGAL STRUCTURE - MOLD
where hyphal structures are interrupted at some points (at regular interval) by partitions or cross walls
Septate