19 - Genetics of living systems Flashcards
What is a mutation?
Change in the base sequence of DNA
What are the 3 types of mutation?
- Substitution 2. Deletion 3. Insertion
What is a substitution mutation?
Replacement of one or more DNA bases with others
What is a deletion mutation?
When one or more nucleotides are removed (i.e., deleted) from the DNA sequence
What is an insertion mutation?
Where one or more bases are added to the DNA sequence
What is it called when a mutation only affects one nucleotide?
Point mutation
When might a mutation affect a protein’s primary structure?
When it creates a new codon which codes for a different amino acid
What are the 3 different types of mutation effects?
- No effect 2. Damaging 3. Beneficial
When would a frameshift mutation occur?
When a deletion or addition mutation occurred in a number of bases that is not a multiple of 3
What would a frameshift mutation cause?
Every successive codon from the point of the mutation onwards would be different, so the protein produced would be completely different
Would a protein still be affected by a deletion or insertion mutation which wasn’t a frameshift mutation?
Yes, as there would still be a new amino acid added, but the effects would likely be less
What are 3 possible reasons why a mutation may have a neutral effect on a protein’s structure?
- Mutation changes a base, but the amino acid which the triplet codes for is the same 2. Mutation causes a different amino acid to be produced which is chemically similar to the original 3. The triplet affected may code for an amino acid which isn’t essential to the protein’s function, such as one located away from the active site
Would a mutation with a neutral effect affect the whole organism?
No it wouldn’t affect the whole organism
How can a mutation make a protein more or less active?
By changing the shape of its active site
Does a mutation which changes a protein’s active site mean the organism is less likely to survive?
Not necessarily- the mutation may even be beneficial
Give an example of a mutation with a beneficial effect for an organism?
A mutation which enabled a bacterial enzyme to break down an antibiotic more effectively
Give an example of a mutation with a negative effect for an organism?
Deletion mutation causing changes in the CFTR protein which lead to cystic fibrosis
How can a mutation prevent a protein from being produced?
If the mutation is at the start of the gene and RNA Polymerase can’t bind to it, so the protein won’t be produced
What are 2 reasons a mutation may be harmful to an organism?
- Protein may not be produced at all 2. Protein may be produced, but in a non-functional form
What increases the rate of mutation?
Mutagens
What is a mutagen?
A chemical, biological or physical agent which causes mutations
What is depurination?
The loss of a purine base from DNA
What is depyrimidination?
Loss of a pyrimidine base from DNA
How does depurination or depyrimidination usually happen?
Spontaneously
What type of mutation could depurination or depyrimidination lead to?
Insertion if a new base filled the gap left
What are 2 chemical mutagens?
- Free radicals 2. Deaminating agents
What could a deaminating agent do to cytosine?
Change it to uracil
Why are antioxidants also known as carcinogens?
Due to their ability to negate the effects of mutagenic free radicals
What is an example of a physical mutagen?
Ionising radiation such as x-rays
How would x-rays act mutagenically?
Break one or both DNA strands, with the repair of the strand often causing mutations
What are 3 examples of biological mutagens?
- Alkylating agents 2. Base analogues 3. Viruses
How does a virus act as a mutagen?
By inserting viral DNA into the genome
How do alkylating agents act as mutagens?
Attach methyl or ethyl groups to bases, causing incorrect pairing during replication
How do base analogues act as mutagens?
They are incorporated into DNA in lieu of a normal base
What is a chromosome mutation?
One which affects the whole chromosome or a number of chromosomes within the cell
When do most chromosome mutations occur?
During meiosis
What is a deletion chromosome mutation?
A section of a chromosome breaks off and is lost within a cell
What is a duplication chromosome mutation?
Sections of a chromosome duplicated
What is a translocation chromosome mutation?
A section of one chromosome breaks off and attaches to a non-homologous chromosome
What is an inversion chromosome mutation?
A section of chromosome breaks off, is reversed, and then rejoins onto the chromosome
What is a housekeeping gene?
One which codes for enzymes necessary for metabolic processes such as respiration
What are tissue-specific genes?
Ones which code for protein-specific hormones
What is a transcription factor?
Proteins which bind to DNA and switch genes on or off by increasing or decreasing the rate of transcription
What are the two types of transcription factor?
- Activators 2. Repressors
What do activators do?
Factors which increase the rate of transcription
What do repressors do?
Factors which decrease the rate of transcription
How do transcription factors work?
Bind to specific DNA sites near the start of their target genes (in eukaryotes) and promote/repress the action of RNA Polymerase
What do transcription factors bind to in prokaryotes?
Operons
What 3 things can be contained in an operon?
- Clusters of structural genes 2. Control elements 3. A regulatory gene (sometimes)
What do structural genes do?
Code for useful proteins i.e. enzymes
What 2 things do control elements contain?
- Promoter 2. Operator
What is a promoter?
DNA located before the structural genes, which RNA polymerase binds to
What is an operator?
A DNA sequence which transcription factors bind to
What 2 things can regulatory genes code for?
Activators or repressor
What allows bacteria to respond to environmental changes?
Gene regulation
What 4 things is gene regulation necessary for in eukaryotes?
- Responding to changes in external environment 2. Responding to changes in internal environment 3. Cell specialisation 4. Cell coordination
What are the 4 stages at which genes can be regulated?
- Transcriptional 2. Post-transcriptional 3. Translational 4. Post-translational
How can genes be regulated at a transcriptional level?
Genes can be turned on or off
How can genes be regulated at a post-transcriptional level?
mRNA can be modified, which regulates translation and the types of proteins produced
How can genes be regulated at a translational level?
Can stop or start translation
How can genes be regulated at a post-translational level?
Proteins can be regulated after translation, which changes their function
What are 4 methods of transcriptional gene regulation?
- Cyclic AMP 2. Histone modification 3. Chromatin remodelling 4. Lac operon
What is a chromatin?
DNA molecules that are tightly coiled around proteins call histones.
What is heterochromatin?
Tightly packed chromatin
When does heterochromatin allow chromosomes to be seen?
During cell division
When does euchromatin allow chromosomes to be seen?
During interphase
What is euchromatin?
Loosely packed chromatin
In which of heterochromatin and euchromatin can genes be transcribed and why?
Euchromatin, as heterochromatin is too tightly packed
Does protein synthesis occur during cell division, and why/why not?
No as you can’t transcribe genes from heterochromatin
Why is it important that genes cannot be transcribed from heterochromatin?
Prevents complex and energy-consuming process of protein synthesis occurring when cells are dividing
What type of gene regulation is the fact that genes can’t be transcribed from heterochromatin?
Transcriptional
Why does DNA condense around histones?
Because they are positively charged and DNA is negatively charged
What are 2 methods of making histone groups less positive?
Acetylation and phosphorylation
How is the acetylation or phosphorylation of a histone an example of transcriptional gene regulation?
Makes DNA bind to histones more loosely, allowing certain genes to be transcribed
How does methylation of histones affect them?
Makes them more hydrophobic
How is methylation of histones an example of transcriptional gene regulation?
Because it makes them more hydrophobic they bind together more tightly, so the DNA coils around them more tightly and less gene transcription can take place