19 Genetic Technology

Question 1

What are the 3 temps needed in PCR?

Answer 1

95, 65, 72

Question 2

What is the purpose of PCR?

Answer 2

to amplify DNA

Question 3

What property of DNA allows small fragments to separate in gel electrophoresis?

Answer 3

DNA negatively charged due to phosphate group

Question 4

what does lacA code for?

Answer 4

acetyl transferase

Question 5

what does lacY code for?

Answer 5

permease

Question 6

what does lacZ code for?

Answer 6

lactase enzyme

Question 7

what are transcription factors?

Answer 7

control gene expression in eukaryotes

Question 8

how is gene transcription affected when giberellin is absent?

Answer 8

PIF can’t bind to promoter (binded to DELLA) so RNA polymerase can’t bind to promoter so the gene is not transcribed

Question 9

what is giberellin?

Answer 9

a hormone

Question 10

what is PIF?

Answer 10

transcription factor

Question 11

how is gene transcription affected when giberellin is present?

Answer 11

giberellin

Question 12

how much atp is produced during glycolysis?

Answer 12

4 atp, but net 2 atp

Question 13

what are three adaptations of rice plants?

Answer 13

1) early rapid shoot growth
2) aerenchyma
3) produce large amounts of ethanol dehydrogenase

Question 14

what are the products of the Krebs Cycle?

Answer 14

1) 2x co2
2) 3x NAD
3) 1x FAD
4) 1x ATP