152 Lab Practical

Question 1

How to store all solutions?

Answer 1

Solutions 1-4 and the unknown are to be quantitatively transferred to labeled french square botteles.

Question 2

Step 1 of calibrating logger pro?

Answer 2

Place solution 1 in a cuvette via quantitative transfer and place this cuvette in the spectrophotometer.

Question 3

Step 2 of calibrating logger pro?

Answer 3

Click Experiment in the top left corner of logger pro, go down to calibrate and select spectrometer.

Question 4

Step 3 of calibrating logger pro?

Answer 4

Allow the light bulb to warm up and then press finish Calibration and ok

Question 5

How to know if the graph has been configured?

Answer 5

the absorbance should read around 0.000 with solution 1 in the spectrophotometer

Question 6

Step 1 in configuring logger pro?

Answer 6

Soultion 2 can be placed in a cuvette via quantatative transfer and then placed in spectrophotometer after having been calibrated with solution 1.

Question 7

Step 1 in configuring logger pro?

Answer 7

Press collect, wait 10 seconds, press stop, then press autoscale graph, analyze and then interpolate.

Question 8

Step 1 in configuring logger pro?

Answer 8

Hover mouse over the peak of the graph to determine the lambda max and record the value

Question 9

What should lambda max be around?

Answer 9

~450nm

Question 10

Where will lambda max be found?

Answer 10

at the peak of the graph.

Question 11

Step 1 in configuring logger pro?

Answer 11

Press the configure spectrophotometer button, which is rainbow and left of the collect button, and press ok

Question 12

What will the graph do once the finishing step has been completed in configuring logger pro?

Answer 12

It will switch to a blank page and the absorbance will be displayed in the bottom left corner.

Question 13

How should the 50 mL vol. flask be cleaned before creating the .001M NaSCN dilution?

Answer 13

With pure water

Question 14

How should the pipette tip be cleaned before pipetting the NaSCN into the 50 mL Vol Flask?

Answer 14

With 0.02 M NaSCN

Question 15

How should the pipette tip be cleaned before pipetting the NaSCN into the 25 mL Vol Flask?

Answer 15

0.001M NaSCN

Question 16

How should the pipette tip be cleaned before pipetting the unknown into the 25 mL Vol Flask?

Answer 16

With Nitric Acid (HNO3)

Question 17

How should the eyedropper be cleaned before creating the 0.001M NaSCN dilution?

Answer 17

With Nitric Acid (HNO3)

Question 18

How should the eyedropper be cleaned before creating the unknown solution?

Answer 18

With Nitric Acid (HNO3)

Question 19

How should the eyedropper be cleaned before remaking any solution?

Answer 19

With Nitric Acid (HNO3)

Question 20

How to prime French Square Bottles before putting any solution of any kind in them?

Answer 20

Prime with whatever solution that is about to go in.

Question 21

How to prime any Cuvettes before adding in any solution?

Answer 21

Prime with whatever solution will be going in it.

Question 22

How to solve for the unknown diluted?

Answer 22

M1V1=M2V2

Question 23

How to solve for the unknown undiluted?

Answer 23

y=mx+b from graph is used.

Absorbance= 4032 x (concentration) +0.0151

Substitute the unknown Absorbance that you find for the Absorbance in the equation.

Then solve for concentration.

Question 24

How to calcultate the Molarity of SNC- in the graph?

Answer 24

Use M1V1=M2V2. M1 and V1 will be the NaSCN and its respective mL. M2 will be what is needed to be found and V2 is the total amount of volume ( every mL added up) which will be around 25 mL