12.2 The Golgi Apparatus Flashcards
there is a distinct () in both the structure and function of the Golgi apparatus
polarity
what are the discrete compartments of the Golgi?
- cis compartment
- medial compartment
- trans compartment
- trans-Golgi network
what occurs in the cis compartment
- proteins from the ERGIC enter the Golgi
- site of protein, lipid, and polysaccharide modification
what happens in the medial compartment
modification of molecules
what happens in the trans compartment
further modification of molecules
what happens in the trans-Golgi network
- sorting and distribution of proteins
- direction of molecular traffic to endosomes, lysosomes, the plasma membrane, or the cell exterior
protein processing within the Golgi involves extensive modification of the (1) of (2)
- carbohydrate portions
- glycoproteins
what are the kinds of protein glycosylation that occur in the Golgi?
- N-linked glycosylation
1.1. N-linked glycosylation of lysosomal proteins - O-linked glycosylation
give an overview of the processing of N-linked oligosaccharides in the Golgi
- in the cis compartment: mannose residues are removed from N-linked oligosaccharides of glycoproteins (from ER); N-acetylglucosamine is added
- in medial compartment: Fucose, N-acetlyglucosamine, and galactose are added
- in trans compartment and trans-Golgi network: sialic acid is added
glycoproteins destined for the lysosomal membrane are modified by (1) that occurs in the (2) of the Golgi
- mannose phosphorylation
- cis compartment
give an overview of the N-linked glycosylation of lysosomal proteins
- N-acetylglucosamine phosphates are transferred from UDP-N-acetylglucosamine to mannose residues (in the #6 position)
- N-acetylglucosamine group is removed
- the resulting mannose-6-phosphate residues remain on the N-linked oligosaccharide
- the phosphorylated mannose residues are specifically recognized by a mannose-6-phosphate receptor in the trans-Golgi network (directs the transport of these proteins to endosomes and on to lysosomes)
the addition of carbohydrates to the side chains of acceptor serine and threonine residues
O-linked glycosylation
give an example of a product of O-linked glycosylation
proteoglycans
brief summary of what happens in O-linked glycosylation
modifications take place in the Golgi apparatus by the sequential addition of single sugar residues
the Golgi is involved in the synthesis of (1) and (2) (from (3) synthesized in ER)
- glycolipids
- sphingomyelin
- ceramide
summarize the synthesis of sphingomyelin in the Golgi
in the lumenal surface of the Golgi, a phosphorylcholine group is transferred from phosphatidylcholine to ceramide
give an overview of glycolipid synthesis in the Golgi
- glucose is added to cermide on the cytosolic side
- glucosylceramide is flipped to the lumenal side and additional carbohydrates are added
note that a variety of glycolipids can be synthesized by the (…)
addition of one or more sugar residues to ceramide
following vesicular transport and fusion to the plasma membrane, the () of the lipids remain exposed on the cell surface
polar head groups
() of glycolipids are important surface markers in cell to cell recognition
oligosaccharide portions
most proteins, lipids, and polysaccharides are transported from the Golgi to their final destinations through the ()
secretory pathway
how are proteins carried to the plasma membrane?
either carried directly to plasma membrane by vesicles or via endosomes (that act as intermediate compartments)
what happens to proteins that function within the Golgi?
- these proteins are usually usually transmembrane proteins (function in glycosylation) and are retained within the Golgi by signals within their membrane-spanning and cytoplasmic domains
- signals in some Golgi proteins mediate their retrieval from compartments along the secretory pathway and transport from endosomes back to the trans-Golgi network
proteins can also be transported to the plasma membrane via (), which also act to recycle endocytosed proteins to the plasma membrane
recycling endosomes
how are regulated proteins secreted in response to environmental signals?
- proteins are sorted into the regulatory secretory pathway (by trans-Golgi network) and are packaged into immature secretory granules
- aggregation of proteins into secretory granules is driven by the proteins’ structural characteristics (selective aggregation in the slightly acidic environment of the trans-Golgi network)
- the proteins are stored in matured secretory granules until they are to be secreted in response to an environmental signal
explain how proteins are targeted to plasma membrane domains of polarized cells (e.g. epithelial)
- proteins leaving the trans-Golgi network of these cells must be selectively packaged into vesicles specifically targeted to either the apical or basolateral domain
- selective packaging occurs in either tGn or recycling endosomes
- proteins are targeted by short animo acid sequences
() between the plasma membrane domains in epithelial cells prevent the diffusion of proteins between the domains
tight junctions
proteins are targeted to lysosomes by ()
mannose-6-phosphate residues