1.2.2(g) separation of biological compounds using thin layer/paper chromatography or electrophoresis

Question 1

what should you do to ensure you don’t gen aberrant results in TLC and chromatography

Answer 1

Chromatography paper and TLC plates should be handled carefully as oils on fingers and hands will cause aberrant results.

Question 2

what kind of spot gives the best results?

Answer 2

small intense spot.In most cases the concentration of the sample in extraction solvent will be so low that multiple spots will need to be superimposed to achieve the required intensity. Ensure that the previous spot has dried before adding the next spot on top of it otherwise the spot will spread out and ultimately give a smudged separation.

Question 3

Unfortunately the best extraction and running solvents often include hazardous organic solvents. what precautions should be taken?

Answer 3

Use a fume cupboard to minimise exposure and ventilate the lab as well as possible. Ensure that the solvents are disposed of correctly after use.