1.2.2(c) use of laboratory glassware apparatus for a variety of experimental techniques to include serial dilutions

Question 1

when to use a serial dilution

Answer 1

. in enzyme reactions or as standards for a calibration curve, and are particularly useful in microbiology when counting the number of bacterial colonies

Question 2

Use of a burette

Answer 2

Before using a burette, it should be rinsed first with distilled water and then with the solution it is going to contain

Question 3

what should you ensure when using a burette?

Answer 3

solution should be added to the burette so it is above the zero mark

and then some of it allowed to run through the tap until the tip of the burette is full of solution and contains no bubbles.

The initial reading on the burette does not need to be exactly 0.00 cm3.

Question 4

what can you use to help put the solution into the burette

Answer 4

A small funnel can be used when solution is added to the burette. A filter funnel is not suitable for this purpose. The funnel should be removed as soon as the solution has been added. T