11.6 Artificial chromosomes

Question 1

What are the three key elements that artificial chromosomes require?

Answer 1

1. Centromeres
2. Telomeres
3. Origins of replication

Question 2

What are the consequences of deleting key elements?

Answer 2

1. No centromere, no telomere – replicate, segregation errors
   No telomere – replicate, degrade if linear