1.1.5 Microscopy 2

Question 1

Tell me about light microscopes.

Answer 1

1st used in 16th century.
Light microscopes pass light through a specimen and creates a magnified image using lenses.

Question 2

What were some of the discoveries using a light microscope.

Answer 2

Allowed us to see bacteria for the first time and distinguish plant and animal cell structures.
But still could not tell lots of sub-cellular structures apart.

Question 3

Tell me about the electron microscope.

Answer 3

1st used in 1933.
Electrons are passed through specimens instead of light.
Can resolve distances of 1nm (better resolution) with magnifications of x500,000

Question 4

What were some of the discoveries using an electron miscroscope?

Answer 4

Allowed scientists to see sub-cellular structures more clearly.
Allowed scientists to study how stuctures such as mitochondria, ribosomes and chloroplasts function.

Question 5

What were some of the discoveries using an electron miscroscope?

Answer 5

Allowed scientists to see sub-cellular structures more clearly.
Allowed scientists to study how stuctures such as mitochondria, ribosomes and chloroplasts function.

Question 6

What is the maximum resolution and magnification that an electron microscope can achieve?

Answer 6

Magnification: x500,000
Resolution: 1nm

Question 7

The ____ microscope was first used in 1933.

Answer 7

electron

Question 8

What is the name of the lens with the lowest magnification that we use to initially focus on a sample?

Answer 8

Objective lens

Question 9

When using a light microscope, use the lowest power ____ lens, and focus on the sample. Increase the power and ____.

Answer 9

Objective
Refocus