10: environment on epigenetics

Question 1

what is folate used for?

Answer 1

normal RBC production

Question 2

how does the circadian clock affect epigenetics?

Answer 2

in the day:
CLOCK-BMAL1 bind to E-boxes -> period, cryptochrome

period and cryptochrome accumulates -> translocates into nucleus at night -> inhibits CLOCK and BMAL1

Question 3

what does H2A.Z do?

Answer 3

a histone variant that is sensitive to temp changes, replaces H2A in nucleosomes

lower temp: wrap DNA more tightly
higher temp: local unwrapping
H2AZ may be more/less stable depending on its histone partner

Question 4

how does H2A.Z get inserted?

Answer 4

ARP6 encodes for a subunit in SWR1 which is needed for H2AZ to replace H2A