10. Bacteria Flashcards
1
Q
List 9 diagnosis of bacteria.
A
- Gram staining
- Nutritional requirement
- Selective media
- Fatty acid analysis
- Pathogenicity inoculated to hosts
- Phage typing
- Serology or immunology
- Molecular detection (based on DNA)
- Bacterosins
2
Q
What is used to make the Fatty acid analysis?
A
Gas chromatography to identify the compounds in fatty acids
3
Q
Which organisms are used for Phage typing?
A
Viruses which specifically lyse bacteria-sensitivity
4
Q
What assay is used for Serology or Immunology diagnosis?
A
ELISA: enzyme linked immuno sorbant assay, where specific antibodies are produced
5
Q
What three technics are used for molecular detection based on DNA?
A
- PCR: polymerase chain reaction
- RAPD: randomly amplified polymorphic DNA
- RFLP: restriction gragment length polymorphism
6
Q
Which organisms does Bacteriocin use? what do they secrete?
A
- Bacteria
- antibiotics which lyse certain bacteria
