10 Flashcards
- encloses
the cell, defines its boundaries, - maintains the essential differences between
the cytosol and the extracellular environment
plasma membrane
maintain the characteristic differences
between the contents of each organelle and the cytosol
membrane-enclosed organelles
Across membranes, it is established by the activities of specialized membrane proteins
- can
be used to synthesize ATP, to drive the transport of selected solutes across the
membrane, or, as in nerve and muscle cells, to produce and transmit electrical
signals
Ion gradients
- plasma membrane also contains proteins that act as sensors of external signals, allowing the cell to change its behavior in response to environmental cues, including signals from other cells
protein sensors, or
receptors
transfer information—rather than molecules—across the membrane
protein sensors, or
receptors
provides the basic fluid structure of
the membrane and serves as a relatively impermeable barrier to the passage of
most water-soluble molecules.
lipid bilayer
span the lipid bilayer and
mediate nearly all of the other functions of the membrane
membrane proteins
provides the basic structure for
all cell membranes
Lipid Bilayer
50% of the mass of the most
animal cell membranes
lipids
All of the lipid molecules in cell
membranes are
amphiphilic
they have a hydrophilic (“water-loving”) or
polar end and a hydrophobic (“water-fearing”) or nonpolar end.
amphiphilic
most abundant membrane lipids
phospholipids
The parts of phospholipid molecule
polar head group (hydrophilic) containing a phosphate group and two hydrophobic hydrocarbon tails
containing a phosphate group
Head
creates kink in the tail
cis-double bonds (unsaturated)
What do cis-double bonds create
kink in the tail
main phospholipids in most animal cell membranes
phosphoglycerides
component of phosphoglycerides
- three-cardon glycerol backbone
- two long-chain fatty acids
- 3rd carbon is attached to a phosphate
group, which is linked to a head group
the most abundant phosphoglycerides in mammalian cell membranes
phosphatidylethanolamine,
phosphatidylserine, and
phosphatidylcholine
- Another important class of phospholipids
- from sphingosine
sphingolipids
sphingolipids are built from
sphingosine
long acyl chain with an amino group (NH2) and two hydroxyl groups (OH)
Sphingosine
- most common sphingolipid
- a fatty acid tail is attached to the
amino group, and a phosphocholine
group is attached to the terminal
hydroxyl group
sphingomyelin
In sphingomyelin, a ____ ___ tail is attached to the amino group, and a ____
group is attached to the terminal
hydroxyl group
fatty acid
phosphocholine
- resemble sphingolipids, but, instead
of a phosphate-linked head group, they have sugars attached
Glycolipids
- sterol
- contains a rigid ring structure, to which attached a single polar hydroxyl group and a
short nonpolar hydrocarbon chain
cholesterol
cholesterol contains?
rigid ring structure
what is attached on the rigid ring structure of cholesterol
single polar hydroxyl group and a short nonpolar hydrocarbon chain
What causes the phospholipid to form bilayers spontaneously in aqueous environments.
shape and amphiphilic nature
spontaneously aggregate to bury their hydrophobic tails in the interior, where they are shielded from the water, and they expose their hydrophilic heads to water.
amphiphilic molecules
2 ways of amphiphilic molecules to bury their hydrophobic tails in the interior, and expose their hydrophilic heads to water.
They form:
1. spherical micelles, ( tails inward)
2. double-layered sheets, or bilayers, (hydrophobic tails sandwiched between the hydrophilic head groups)
Parts of cholesterol
polar head groups
Cholesterol -stiffened region
More fluid region
same forces that drive phospholipids to form bilayers also provide a ___-___ ____
self-sealing property
creates a free edge with water;
because this is energetically unfavorable, the lipids tend to rearrange spontaneously to eliminate the free edge
small tear in the bilayer
Energetically unfavorable
Planar phospholipid bilayer with edges exposed to water
Energetically favorable
sealed compartment formed by phospholipid bilayer
only way for a bilayer to avoid having edges
closing in on itself and forming a
sealed compartment
are able to diffuse freely within the plane of a lipid bilayer
lipid molecules
have been used to measure the motion of individual lipid molecules and their components
Various techniques
- can be use to construct a lipid molecule
a fluorescent dye or small gold
particle attached to its polar group
one can modify a lipid head group to carry a ___ ___
spin label
Example of a “spin label”
nitroxide group (=N–O)
contains an unpaired electron whose spin creates a paramagnetic signal that can be
detected by electron spin resonance (ESR) spectrum
nitroxide group (=N–O)
phospholipid molecules in synthetic bilayers very rarely migrate from the monolayer on one side to that on the other
flip-flop”
lipid molecules rapidly exchange
places with their neighbors within a
monolayer
rapid lateral diffusion
individual lipid molecules rotate very rapidly about their ___ ___ and have flexible ____ chains
long axis
hydrocarbon
the lipid component of a biological membrane
two-dimensional liquid
constituent molecules are free to move laterally due to
two-dimensional liquid
catalyzes the rapid flipflop of phospholipids from one monolayer to the other
phospholipid translocators
(flippases)
Mobility of phospholid
- lateral diffusion
- Flip-flop (rarely occurs)
- Rotation
- Flexion
a change from a liquid state to a two-dimensional rigid crystalline state at a
characteristic temperature
phase transition
modulates the properties of lipid bilayers
cholesterol
When mixed with phospholipids, what does cholesterol enhances
permeability-barrier
properties
What does cholesterol decrease that makes the lipid less deformable
mobility of the first few CH2
groups of the chains of phospholipid molecules
what happens when lipid bilayer is less deformable
decreases the permeability of the bilayer to small water-molecule
What does cholesterol prevent
the hydrocarbon chains from
coming together and crystallizing
lipids usually contain 20–25-carbon-long prenyl chains
archaea
what does lipid contain in archaea
20–25-carbon-long prenyl chain
can be built from molecules with similar features but different molecular designs.
lipid bilayers
lipid bilayers can be built from molecules with
similar ____ but different ____
designs
features
molecular
more varied than those of prokaryotes and
archaea
plasma membranes of most eukaryotic cells
eukaryotic cell membrane has how mamy different lipid species
500-200
specialized membrane microdomains that
serve as to organizing centers for assembly of signaling molecules, influence membrane fluidity and trafficking of membrane proteins, and regulate different cellular processes such as neurotransmission and receptor
trafficking
lipid rafts
function of lipid raft
- organizing centers for assembly of signaling molecules
- influence membrane fluidity and trafficking of membrane proteins
- regulate different cellular processes such as neurotransmission and receptor
trafficking
specific lipids come together in
separate domains
phase segregation
- storage of lipids
- are surrounded by a single
monolayer of phospholipids, which contains a large variety of proteins - form rapidly when cells are exposed to high concentrations of fatty acid
lipid droplets
- specialized for lipid storage
- contain a giant liquid droplet
fat cells or adipocytes
fat cells or adipocytes contains
a giant liquid droplet