1. Photosynthesis and ATP synthesis 2 Flashcards
Explain cyclic photophosphorylation
Light is absorbed by PSI
light energy is passed on to electrons and excites them in the chlorophyll a molecules at the reaction centre.
In each chlorophyll a molecule, one of the electrons becomes so energetic that it moves to a higher energy level and leaves the chlorophyll molecules completely.
The electron is then passed along the chain of electron carriers (e transport system)
The energy from the electron is used to make ATP.
The electron, now having lost its extra energy, eventually returns to chlorophyll a in PSI.
The electron transport system consists of a series of carrier molecules and, as electrons pass from one carrier molecule to the next, energy is released.
This energy release causes
H+ to be actively pumped into the thylakoid from the stroma across the thylakoid membrane.
The concentration of H+ inside the thylakoid space is therefore higher than in the stroma outside.
Thus, a H+ gradient is created across the thylakoid membrane.
The H+ can diffuse back across the thylakoid membrane and into the stroma only via specific protein channels called chemiosmotic channels.
As H+ flow from inside the thylakoid membrane to the outside of the thylakoid down their electrochemical gradient (proton gradient), energy is generated to drive the enzymatic conversion of ADP and P (inorganic phosphate found in the cytosol) into ATP.
The reactions of the Calvin cycle that occur in the stroma require a larger amount of ATP than NADPH and this is where the ATP is used up.
Chemiosmotic channels are formed by
enzyme complexes called ATP synthase
Non-cyclic photophosphorylation involves what type of photosystem
both kinds of photosystem.
PSI and PSII
Non-cyclic photophosphorylation results in
the production of ATP and NADP
what are the three stages of Non-cyclic photophosphorylation
1 ) Photolysis of water produces protons (H+ ions), electrons and 0 2
2) Energy from the excited electrons makes ATP…
3) reduced NADP is generated
explain the first stage of Non-cyclic photophosphorylation where Photolysis of water produces protons (H+ ions), electrons and O2 takes place
The chlorophyll a molecules of PSII absorb light energy
the excited, high energy electron from chlorophyll leaves the reaction centre chlorophyll a molecule of PSII (and moves down the electron chain-photophosphorylation)
As soon as the high-energy electrons leave the reaction centre, PSII immediately takes up replacement electrons from a water molecule
the water molecule splits into two electrons, two H+ (protons) and an oxygen atom. (two oxygen atoms from two water molecules combine to form a molecule of oxygen)
The H+ stay inside the thylakoid space and contribute to the formation of a proton gradient which is used to generate ATP.
the reaction for this is
2H2O → 4H+ + 4e− + O2
The light-dependent splitting of water molecules is called
photolysis
Photolysis is
chemical process by which molecules are broken down into smaller units through the absorption of light
the light-dependent splitting of water molecules
Process by which light energy breaks down a molecule
explain the second stage of Non-cyclic photophosphorylation where energy from the excited electrons makes ATP…
High-energy electrons from PSII are taken up by an electron acceptor, which transfers them to an electron transport system.
The electron transport system consists of a series of molecules that act as electron carriers and as the electrons are transported along,
energy is released and used to transport H+ into the thylakoid and stored in the form of a proton gradient across the thylakoid membrane
The production of ATP occurs when the H+ inside the thylakoid flow down their electrochemical gradient to the outside of the thylakoid (the stroma) via the ATP synthase (which is embedded in the thylakoid membrane)
the energy of this movement combines ADP an inorganic phosphate (Pi) to form ATP
(Chemiosmosis-production of ATP using an electrochemical gradient)
Chemiosmosis is
the process by which a Hydrogen pump pumps protons into the thylakoid membrane.
The production of ATP using an electrochemical gradient
H+ passively flows through the ATP synthase which leads to the creation of ATP.
explain the third stage of Non-cyclic photophosphorylation where reduced NADP is generated
After the high-energy electrons have travelled along the electron transport system they have lost energy and become low-energy electrons.
The low-energy electrons enter PSI.
The PSI antenna complex absorbs solar energy and the high-energy excited electrons leave the reaction centre chlorophyll a
then the electrons are taken up by an electron acceptor, which passes them to NADP. The electrons, NADP and H+ bind and become NADPH
Photosystem I uses light energy to
reduce NADP to NADPH
Photosystem II uses light energy to
oxidize water molecules to form oxygen (O2 ), H+ and electrons
the non-cyclic electron pathway starts with
PSII
the only mechanism by which ATP is generated during photosynthesis is by
the proton gradient across the thylakoid membrane
Cyclic photophosphorylation only uses PSI. It’s called ‘cyclic’ because
the electrons from the chlorophyll molecule aren’t passed onto NADP, but are passed back to PSI via electron carriers.
This means the electrons are recycled and can repeatedly flow through PSI.
This process doesn’t produce any reduced NADP or O , — it only produces small amounts of ATP
Both cyclic and non-cyclic photophosphorylation stop
when
there is no light because ATP synthesis and NADP
reduction (i.e. production of NADPH) require light
The rates of cyclic and non-cyclic photophosphorylation depend on
each other because they are linked by the exchange of ATP and ADP and NADP and NADPH with the Calvin cycle and photosystems
The Z-scheme is
simply a way of summarising what happens to electrons during the light dependent reactions.
It is a kind of graph, with the y-axis indicating the ‘energy level’ of the electron
explain the Z-scheme, summarising non-cyclic photophosphorylation
1) light hits photosystem II. The red vertical line going up shows the increase in the energy level of electrons as they are emitted from this photosystem.
2) If you keep following the vertical line showing the increasing energy in the electrons, you arrive at a point where it starts a steep dive downwards. This shows the electrons losing their energy as they pass along the electron carrier chain.
3) Eventually they arrive at photosystem I.
4) You can then track the movement of the electrons to a higher energy level when PSI is hit by light, before they fall back downwards as they lose energy and become part of a reduced NADP molecule.
the electrons in the z scheme come from
the splitting of water molecules
The thylakoid membrane contains several protein complexes that perform different functions associated with photosynthesis.
The protein complexes are:
PSI is a protein complex in association with the antenna complex of chlorophyll a molecules and the enzyme that reduces NADP to NADPH
PSII is a protein complex in association with the antenna complex of chlorophyll a molecules.
PSII splits water to give oxygen and H+ that contribute to electrochemical gradient across the thylakoid membrane.
ATP synthase complex has an ion channel that allows H+ to flow down their electrochemical gradient from the inside of the thylakoid space to outside in the stroma thereby generating ATP from ADP.
A cytochrome is
a protein that contains a haem group that serves as electron carriers in respiration and photosynthesis
A cytochrome complex transports electrons from PSII and PSI.
A cytochrome complex transports
electrons from PSII and PSI.
haem is an
ion-containing prosthetic group such as that in haemoglobin
The Calvin cycle takes place in
the stroma of the chloroplasts
Products of Calvin Cycle
triose phosphate from CO2 (used to make glucose and other useful organic substances)
ribulose bisphosphate (a 5-carbon compound)
Calvin cycle needs what to keep it going?
ATP and H+ ions
The Calvin cycle is also known as carbon dioxide fixation because
carbon from CO2 is ‘fixed’ into an organic molecule.
Carbon enters the calvin cycle as ___ and leaves as ___
Carbon enters the Calvin cycle as carbon dioxide and leaves as sugar
The fixation of carbon dioxide is the
conversion of carbon dioxide to an organic compound
The Calvin cycle (Light independent stage) can be divided into three basic steps
1) Carbon Fixation
2) Reduction of Glycerate-3-Phosphate
3) Regeneration of RuBP
the Calvin cycle is where
the now ATP and reduced NADP that have been formed in the light-dependent stage are used to help to produce carbohydrates from carbon dioxide.
explain what happens in the first stage of the Calvin cycle where carbon fixation takes place
CO2 enters the leaf through the stomata and diffuses into the stroma of the chloroplast.
Here, it’s combined with ribulose bisphosphate (RuBP), a 5-carbon compound. This reaction is catalysed by the enzyme rubisco. •
This gives an unstable 6-carbon compound, which quickly breaks down into two molecules of a 3-carbon compound called glycerate 3-phosphate(GP)/ 3-phosphoglycerate(PGA)
What is the first product of the Calvin cycle
3-phosphate(GP)/ 3-phosphoglycerate(PGA)
glycerate 3-phosphate(GP) is also called
3-phosphoglycerate(PGA)
The chloroplast stroma contains an enzyme called
rubisco
Rubisco full name is
Ribulose-1,5-bisphosphate carboxylase
what is thought to be the most abundant enzyme in the world
Rubisco
explain what happens in the second stage of the Calvin cycle where the Reduction of Glycerate-3-Phosphate takes place
The hydrolysis of ATP (from the light dependent reaction) provides energy to turn the 3-carbon compound, GP, into a different 3-carbon compound called triose phosphate (TP).
this reaction also requires ATP to supply the energy and H+ ions, which come from reduced NADP (also from the light-dependent reaction).
Reduced NADP is recycled to NADP.
Some triose phosphate is then converted into useful organic compounds (e.g. glucose) and some continues in the Calvin cycle to regenerate RuBP
what is the first carbohydrate that is made in photosynthesis
triose phosphate (TP)
RuBP molecules each contain
five atoms of carbon
explain what happens in the third stage of the Calvin cycle where the Regeneration of RuBP takes place
Five out of every six molecules of TP produced in the cycle aren’t used to make hexose sugars, but to regenerate RuBP.
energy for this reaction is supplied by ATP
Regenerating RuBP uses the rest of the ATP produced by the light-dependent reaction.
diagram showing the Calvin cycle
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Function of Rubisco
to catalyse the reaction in which carbon dioxide combines with a substance called RuBP(ribulose bisphosphate).
Rubisco is
a plant enzyme involved in the light independent stage of photosynthesis which catalyzes the fixing of atmospheric carbon dioxide during photosynthesis by catalyzing the reaction between carbon dioxide and RuBP
the enzyme that fixes the carbon dioxide is
Ribulose bisphosphate carboxylase (RuBP carboxylase
RuBP carboxylase has a relatively low enzymatic rate because it
catalyses the fixation of a few molecules of its substrate (carbon dioxide) per second
In a cycle, the starting molecule must be
regenerated in order for the cycle to continue