1. Intro

Question 1

Ideal weight of the stool specimen

Answer 1

5 g

Question 2

Liquid/near-liquid stool specimens should be examined within _________ to ______________ (reason)

Answer 2

30 minutes of collection; to preserve motile trophozoites

Question 3

Soft specimens must be examined within ___________

Answer 3

60 minutes of collection

Question 4

Formed specimens must be processed within ___________

Answer 4

24 hours (may be refrigerated)

Question 5

2 preparations of preservatives used in Parasitology

Answer 5

• Two-vial system: formalin/polyvinyl alcohol (PVA)

- One-vial system: sodium acetate formalin (SAF)

Question 6

Ideal ration of fixative to stool

Answer 6

3 parts fixative, 1 part stool

Question 7

Preservative with mercury

Answer 7

PVA

Question 8

Mercury-free fixative

Answer 8

SAF

Question 9

Fixative for concentration methods and iodine-stained mounts

Answer 9

5-10% formalin

Question 10

Fixative for concentration methods, trichome and other permanent stains

Answer 10

PVA

Question 11

Fixative for concentration methods and permanent stains

Answer 11

SAF

Question 12

What are concentration the methods?

Answer 12

Sedimentation: formalin-ethyl acetate
Flotation: zinc sulfate (sp. gr. 1.18-1.20)

Question 13

Stain for intestinal protozoa

Answer 13

Iron hematoxylin

Question 14

Stain for coccidian protozoa

Answer 14

Modified acid-fast

Question 15

Describe the routine stool sample collection

Answer 15

3 specimens collected every other day within 10 days