1 Cell Biology - Microcopy Flashcards

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1
Q

How do we calculate the total magnification of a microscope?

A

Eyepiece lens x Objective lens magnifying power

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2
Q

State the equation that links magnification, image size and actual size.

A

Image Size = Actual Size x Magnification

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3
Q

Define ‘resolution’.

A

The ability to distinguish between two separate points that are very close together

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4
Q

Which type of microscope has higher magnification and resolving power - Light or electron?

A

Electron microscopes

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5
Q

Why does the tissue sample need to be stained before looking at it under the microscope?

A

To visualise/see structures clearly

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6
Q

Which objective lens should we start using?

A

Low power objective lens

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7
Q

Which focus knob should be used during low and medium power magnification?

A

Coarse focus knob

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8
Q

Which focus knob should be used during high power magnification?

A

Fine focus knob

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9
Q

Why must we not use the coarse focus knob during high power magnification?

A

It moves the stage by too much, may break the lens and the slide

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10
Q

What is the function of a scale bar?

A

To allow us to calculate the actual size of the sample using I=AM

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11
Q

Convert 5mm to um.

A

5000um

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