نظري Flashcards
grow in very salty water. Aerobic, can be autotrophic
Halophiles
growth in very hot temperature, e.g. hot springs,
anaerobes
Thermophiles
Gram Staining, Shapes, arrangements, motility
Microscopic morphology
colony appearance, motility
Macroscopic morphology
aerobic, anaerobic, photosynthetic, growth on selective media
Physiological / biochemical characteristics
e.g.peptides and lipids in cell membranes
Chemical analysis
which phage infects the bacterium
Phage Typing
what antibodies are produced
against the bacterium
Serological analysis
what diseases does the bacterium cause.
Pathogenicity
G + C base composition
DNA analysis using genetic probes Nucleic acid sequencing & rRNA analysis
Genetic & molecular analysis
anaerobes, produce methane as waste product, sewage, landfills, swamps
Methanogens
Are prokaryotes
Can be autotrophs and heterotrophs
Eubacteria
require oxygen to grow (mycobacteria)
obligate aerobes
only grow in absence of oxygen (clostridium tetanus)
obligate anaerobes:
can grow in oxygen and without oxygen (Enterobacteriaceae)
facultative anaerobes:
require low concentrations of oxygen E.g. Neisseria gonorrhoeae
microaerophilic aerobes (microaerophiles):
grows better in absence of oxygen
aerotolerant anaerobes:
– contains bile
– selects for the Enterobacteriaceae
MacConkey agar:
– contains colistin and nalidixic acid
– selects for Staphylococci and Streptococci.
CAN blood agar:
Selective media:
Sodium azide
selects for gram-positive bacteria over gram-negative bacteria
-Bile salts (eg, sodium deoxycholate)—
Acute illness due to the presence of pathogens or their toxins in the blood
Septicemia
Systemic inflammatory response syndrome (SIRS
Sepsis